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Characterization of Bacillus megaterium, Bacillus pumilus, and Paenibacillus polymyxa isolated from a Pinot noir wine from Western Washington State

机译:从西华盛顿州黑比诺葡萄酒中分离出的巨大芽孢杆菌,短芽孢杆菌和多粘芽孢杆菌的特性

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摘要

This report provides the first confirmed evidence of Bacillus-like bacteria present in a wine from Washington State. These bacteria were isolated from a 2013 Pinot noir wine whose aroma was sensorially described as being 'dirty' or 'pond scum.' Based on physiological traits and genetic sequencing, three bacterial isolates were identified as Bacillus megaterium (strain NHO-1), Bacillus pumilus (strain NHO-2), and Paenibacillus polymyxa (strain NHO-3). These bacteria grew in synthetic media of low pH (pH 3.5) while some survived ethanol concentrations up to 15% v/v. However, none tolerated molecular SO_2 concentrations >0.4 mg/1. Growth of strains NHO-1 and NHO-3 in a Merlot grape juice resulted in increases of titratable and volatile acidities while decreases in titratable acidity were noted for NHO-2.
机译:该报告首次证实了华盛顿州葡萄酒中存在芽孢杆菌样细菌。这些细菌是从2013年的黑比诺葡萄酒中分离出来的,该葡萄酒的香气在感觉上被描述为“脏”或“浮渣”。根据生理特征和基因测序,鉴定出三种细菌分离株为巨大芽孢杆菌(菌株NHO-1),短小芽孢杆菌(菌株NHO-2)和多粘芽孢杆菌(菌株NHO-3)。这些细菌在低pH值(pH 3.5)的合成培养基中生长,而有些细菌在乙醇浓度高达15%v / v的条件下仍能存活。但是,没有一个耐受的分子SO_2浓度> 0.4 mg / 1。 Merlot葡萄汁中NHO-1和NHO-3菌株的生长导致可滴定和挥发性酸度增加,而NHO-2的可滴定酸度下降。

著录项

  • 来源
    《Food microbiology》 |2017年第10期|11-16|共6页
  • 作者单位

    School of Food Science, Washington State University, Pullman, WA 99164-6376, USA;

    Formerly South Seattle Community College, Seattle, WA 98106, USA;

    Department of Horticulture, Washington State University, Pullman, WA 99196-6414, USA;

    Department of Horticulture, Washington State University, Pullman, WA 99196-6414, USA;

    School of Food Science, Washington State University, Pullman, WA 99164-6376, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Bacillus; Paenibacillus; Wine; Spoilage;

    机译:芽孢杆菌芽孢杆菌;葡萄酒;损坏;

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