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首页> 外文期刊>Food microbiology >Tolerance development in Listeria monocytogenes-Escherichia coli dual-species biofilms after sublethal exposures to pronase-benzalkonium chloride combined treatments
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Tolerance development in Listeria monocytogenes-Escherichia coli dual-species biofilms after sublethal exposures to pronase-benzalkonium chloride combined treatments

机译：亚致死性暴露于链霉菌蛋白酶-苯扎氯铵联合处理后单核细胞增生李斯特菌-大肠杆菌双物种生物膜的耐受性发展

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This study was designed to assess the effects that sublethal exposures to pronase (PRN) and benzalko-nium chloride (BAC) combined treatments have on listeria monocytogenes-Escherichia coli dual-species biofilms grown on stainless steel in terms of tolerance development (TD) to these compounds. Additionally, fluorescence microscopy was used to observe the changes of the biofilm structure. PRN-BAC exposure was carried out using three different approaches and TD was evaluated treating biofilms with a final 100 μg/ml PRN followed by 50 μg/ml BAC combined treatment. Results showed that exposure to PRN-BAC significantly decreased the number of adhered L monocytogenes (P < 0.05), while E. coli counts remained generally unaltered. It was also demonstrated that the incorporation of recovery periods during sublethal exposures increased the tolerance of both species of the mixed biofilm to the final PRN-BAC treatment. Moreover, control biofilms became more resistant to PRN-BAC if longer incubation periods were used. Regardless of the treatment used, log reduction values were generally lower in L. monocytogenes compared to £ coli. Additionally, microscopy images showed an altered morphology produced by sublethal PRN-BAC in exposed L. monocytogenes-E. coli dual-species biofilms compared to control samples. Results also demonstrated that L. monocytogenes-E. coli dual-species biofilms are able to develop tolerance to PRN-BAC combined treatments depending on way they have been previously exposed. Moreover, they suggest that the generation of bacterial tolerance should be included as a parameter for sanitation procedures design.
机译：这项研究旨在评估亚致死性暴露于链霉蛋白酶（PRN）和苯扎氯铵（BAC）联合处理对单核细胞增生李斯特菌-大肠杆菌双物种生物膜在不锈钢上的耐受性发展（TD）的影响。这些化合物。另外，使用荧光显微镜观察生物膜结构的变化。使用三种不同方法进行PRN-BAC暴露，并评估TD处理生物膜的最终100μg/ ml PRN，然后进行50μg/ ml BAC联合处理。结果表明，暴露于PRN-BAC可以显着减少粘附的L单核细胞增生李斯特菌的数量（P <0.05），而大肠杆菌计数通常保持不变。还证明了在亚致死暴露期间恢复期的纳入提高了混合生物膜的两种物种对最终PRN-BAC处理的耐受性。此外，如果使用更长的孵育时间，对照生物膜对PRN-BAC的抵抗力也将增强。无论采用何种处理方法，单核细胞增生李斯特菌的对数减少值通常均低于大肠杆菌。另外，显微镜图像显示了在暴露的单核细胞增生李斯特菌中，亚致死性PRN-BAC产生的形态发生了改变。大肠杆菌双物种生物膜与对照样品的比较。结果还证明了单核细胞增生李斯特氏菌。大肠杆菌双物种生物膜能够对PRN-BAC联合处理产生耐受性，具体取决于它们先前暴露的方式。此外，他们建议应将细菌耐受性的产生作为卫生程序设计的一个参数。

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