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Enrichment of Acinetobacter spp. from food samples

机译：不动杆菌属的富集。从食物样本

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摘要

Relatively little is known about the role of foods in the chain of transmission of acinetobacters and the occurrence of different Acinetobacter spp. in foods. Currently, there is no standard procedure to recover acinetobacters from food in order to gain insight into the food-related ecology and epidemiology of acinetobacters. This study aimed to assess whether enrichment in Dijkshoorn enrichment medium followed by plating in CHROMagar™ Acinetobacter medium is a useful method for the isolation of Acinetobacter spp. from foods. Recovery of six Acinetobacter species from food spiked with these organisms was compared for two selective enrichment media (Baumann's enrichment and Dijkshoorn's enrichment). Significantly (p ＜ 0.01) higher cell counts were obtained in Dijkshoorn's enrichment. Next, the Dijkshoorn's enrichment followed by direct plating on CHROMagar™ Acinetobacter was applied to detect Acinetobacter spp. in different foods. Fourteen different presumptive acinetobacters were recovered and assumed to represent nine different strains on the basis of REP-PCR typing. Eight of these, strains were identified by rpoB gene analysis as belonging to the species Acinetobacter johnsonii, Acinetobacter calcoaceticus, Acinetobacter guillouiae and Acinetobacter gandensis. It was not possible to identify the species level of one strain which may suggests that it represents a distinct species.
机译：人们对食物在不动杆菌传播链中的作用以及不同不动杆菌属的发生知之甚少。在食物中。当前，没有标准的程序可以从食物中回收不动细菌，以深入了解与食物相关的不动细菌的生态学和流行病学。这项研究旨在评估在Dijkshoorn富集培养基中富集，然后在CHROMagar™不动杆菌培养基中进行铺板是否是分离不动杆菌属的有用方法。从食物。比较了从掺有这些生物的食物中回收的六个不动杆菌属菌种的两种选择性富集培养基（鲍曼氏富集培养基和迪克绍恩富集菌）。 Dijkshoorn的浓缩显着提高了细胞计数（p ＜0.01）。接下来，通过Dijkshoorn富集，然后直接在CHROMagar™不动杆菌上进行铺板，以检测不动杆菌属。在不同的食物中。回收了十四种不同的推定不动杆菌，并根据REP-PCR分型假定为代表九种不同的菌株。通过rpoB基因分析鉴定了其中的八种菌株，它们属于约翰逊不动杆菌，钙乙酸不动杆菌，guillouiae不动杆菌和甘地不动杆菌。无法确定一个菌株的物种水平，这可能表明该菌株代表着不同的物种。

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  • 来源
    《Food microbiology》 |2016年第5期|123-127|共5页
  • 作者

    Ana Carvalheira; Vania Ferreira; Joana Silva; Paula Teixeira;

  • 作者单位

    CBQF - Centro de Biotecnologia e Quimica Fina - Laboratorio Associado, Escola Superior de Biotecnologia, Universidade Catolica Portuguesa/Porto, Rua Arquiteto Lobao Vital, Apartado 2511, 4202-401 Porto, Portugal;

    CBQF - Centro de Biotecnologia e Quimica Fina - Laboratorio Associado, Escola Superior de Biotecnologia, Universidade Catolica Portuguesa/Porto, Rua Arquiteto Lobao Vital, Apartado 2511, 4202-401 Porto, Portugal;

    CBQF - Centro de Biotecnologia e Quimica Fina - Laboratorio Associado, Escola Superior de Biotecnologia, Universidade Catolica Portuguesa/Porto, Rua Arquiteto Lobao Vital, Apartado 2511, 4202-401 Porto, Portugal;

    CBQF - Centro de Biotecnologia e Quimica Fina - Laboratorio Associado, Escola Superior de Biotecnologia, Universidade Catolica Portuguesa/Porto, Rua Arquiteto Lobao Vital, Apartado 2511, 4202-401 Porto, Portugal;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Acinetobacter spp. detection; Enrichment; Selective growth; Food matrix;

    机译：不动杆菌属检测;丰富;选择性增长;食物基质;

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