• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Food microbiology >Phaeobacter inhibens as biocontrol agent against Vibrio vulnificus in oyster models
【24h】

Phaeobacter inhibens as biocontrol agent against Vibrio vulnificus in oyster models

机译：牡蛎模型中Phieobacter inhibens作为对抗创伤弧菌的生物防治剂

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Molluscan shellfish can cause food borne diseases and here we investigated if addition of Vibrio-antagonising bacteria could reduce Vibrio vulnificus in model oyster systems and prevent its establishment in live animals. Phaeobacter inhibens, which produces an antibacterial compound, tropodithietic acid (TDA), inhibited Ⅴ. vulnificus as did pure IDA (MIC of 1-3.9 μM). P. inhibens DSM 17395 (at 105 cfu/ ml) eradicated 10~5 cfu/ml Ⅴ. vulnificus CMCP6 (a rifampicin resistant variant) from a co-culture oyster model system (oyster juice) whereas the pathogen grew to 10~7 cfu/ml when co-cultured with a TDA negative Phaeobacter mutant. P. inhibens grew well in oyster juice to 10~8 CFU/ml and sterile filtered samples from these cultures were inhibitory to Vibrio spp. P. inhibens established itself in live European flat oysters (Ostrea edulis) and remained at 10~5 cfu/g for five days. However, the presence of P. inhibens could not prevent subsequently added Ⅴ. vulnificus from entering the live animals, likely because of too low levels of the biocontrol strain. Whilst the oyster model studies provided indication that P. inhibens DSM 17395 could be a good candidate as biocontrol agent against Ⅴ. vulnificus further optimization is need in the actual animal rearing situation.
机译：软体动物贝类可以引起食源性疾病，在这里我们研究了添加拮抗弧菌的细菌是否可以减少模型牡蛎系统中的弧菌弧菌并阻止其在活体动物中建立。产生抗菌化合物对二硫代乙酸（TDA）的Phieobacter inhibens可抑制Ⅴ。纯IDA（MIC为1-3.9μM）。 P. inhibens DSM 17395（以105 cfu / ml的浓度消灭）10〜5 cfu / mlⅤ。从共培养牡蛎模型系统（牡蛎汁）中提取出的vulnificus CMCP6（一种耐利福平的变体），而与TDA阴性Phaeobacter突变体共培养时，病原体生长至10〜7 cfu / ml。牡蛎汁中的P. inhibens生长良好，达到10〜8 CFU / ml，来自这些培养物的无菌过滤样品对弧菌有抑制作用。 Inhibens P. inhibens在欧洲活的牡蛎（Ostrea edulis）中定居，并以10〜5 cfu / g的价格保持了五天。然而，P。inhibens的存在不能阻止随后添加的Ⅴ。可能由于生物防治菌株的水平过低而导致创伤动物进入活体动物。牡蛎模型研究表明，P。inhibens DSM 17395可能是对抗Ⅴ的良好生物防治剂。在实际的动物饲养情况下，需要进一步优化创伤。

著录项

  • 来源
    《Food microbiology》 |2016年第8期|63-70|共8页
  • 作者

    Cisse Hedegaard Porsby; Lone Gram;

  • 作者单位

    Department of Systems Biology, Technical University of Denmark Matematikorvet, bldg. 301, DK-2800, Kgs. Lyngby, Denmark,Biogen (Denmark) Manufacturing ApS, Biogen IdeC Alle 1, DK-3400 Hillerod, Denmark;

    Department of Systems Biology, Technical University of Denmark Matematikorvet, bldg. 301, DK-2800, Kgs. Lyngby, Denmark;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Vibrio vulnificus; Phaeobacter inhibens; Tropodithietic acid; Oysters; Ostrea edulis; Depuration;

    机译：菌;细菌检查;对二硫辛酸;生蚝;紫菜净化;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号