• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Food microbiology >An individual-based modeling approach to simulate the effects of cellular nutrient competition on Escherichia coli K-12 MG1655 colony behavior and interactions in aerobic structured food systems
【24h】

An individual-based modeling approach to simulate the effects of cellular nutrient competition on Escherichia coli K-12 MG1655 colony behavior and interactions in aerobic structured food systems

机译：基于个体的建模方法来模拟细胞营养竞争对有氧结构食物系统中大肠杆菌K-12 MG1655菌落行为和相互作用的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Traditional kinetic models in predictive microbiology reliably predict macroscopic dynamics of planktonically-growing cell cultures in homogeneous liquid food systems. However, most food products have a semi-solid structure, where microorganisms grow locally in colonies. Individual colony cells exhibit strongly different and non-normally distributed behavior due to local nutrient competition. As a result, traditional models considering average population behavior in a homogeneous system do not describe colony dynamics in full detail. To incorporate local resource competition and individual cell differences, an individual-based modeling approach has been applied to Escherichia coli K-12 MG1655 colonies, considering the micro-bial cell as modeling unit. The first contribution of this individual-based model is to describe single colony growth under nutrient-deprived conditions. More specifically, the linear and stationary phase in the evolution of the colony radius, the evolution from a disk-like to branching morphology, and the emergence of a starvation zone in the colony center are simulated and compared to available experimental data. These phenomena occur earlier at more severe nutrient depletion conditions, i.e., at lower nutrient diffusivity and initial nutrient concentration in the medium. Furthermore, intercolony interactions have been simulated. Higher inoculum densities lead to stronger intercolony interactions, such as colony merging and smaller colony sizes, due to nutrient competition. This individual-based model contributes to the elucidation of characteristic experimentally observed colony behavior from mechanistic information about cellular physiology and interactions.
机译：预测微生物学中的传统动力学模型可以可靠地预测均质液体食品系统中浮游生长的细胞培养物的宏观动力学。但是，大多数食品具有半固体结构，其中微生物在菌落中局部生长。由于局部营养竞争，单个集落细胞表现出非常不同且非正态分布的行为。结果，考虑均质系统中平均种群行为的传统模型并未详细描述菌落动态。为了合并本地资源竞争和个体细胞差异，已将基于个体的建模方法应用于大肠杆菌K-12 MG1655菌落，并将微生物细胞作为建模单位。这种基于个人的模型的第一个贡献是描述了营养缺乏条件下的单菌落生长。更具体地说，模拟了菌落半径演变的线性和固定相，从盘状到分支形态的演变以及菌落中心饥饿区的出现，并与可用的实验数据进行了比较。这些现象在更严重的养分消耗条件下，即在较低的养分扩散率和培养基中的初始养分浓度下，更早地发生。此外，殖民间的相互作用已被模拟。由于养分竞争，较高的接种密度会导致较强的菌落间相互作用，例如菌落合并和菌落尺寸变小。这种基于个体的模型有助于从有关细胞生理学和相互作用的机械信息中阐明实验观察到的菌落行为特征。

著录项

  • 来源
    《Food microbiology》 |2015年第febaptab期|179-188|共10页
  • 作者

    Ignace L.M.M. Tack; Filip Logist; Estefania Noriega Fernandez; Jan F.M. Van Impe;

  • 作者单位

    BioTeC - Chemical and Biochemical Process Technology and Control, Department of Chemical Engineering, KU Leuven, Willem de Croylaan 46 Box 2423, B-3001 Leuven, Belgium,CPMF~2 - Flemish Cluster Predictive Microbiology in Foods OPTEC - Center of Excellence Optimization in Engineering;

    BioTeC - Chemical and Biochemical Process Technology and Control, Department of Chemical Engineering, KU Leuven, Willem de Croylaan 46 Box 2423, B-3001 Leuven, Belgium,CPMF~2 - Flemish Cluster Predictive Microbiology in Foods OPTEC - Center of Excellence Optimization in Engineering;

    BioTeC - Chemical and Biochemical Process Technology and Control, Department of Chemical Engineering, KU Leuven, Willem de Croylaan 46 Box 2423, B-3001 Leuven, Belgium,CPMF~2 - Flemish Cluster Predictive Microbiology in Foods OPTEC - Center of Excellence Optimization in Engineering;

    BioTeC - Chemical and Biochemical Process Technology and Control, Department of Chemical Engineering, KU Leuven, Willem de Croylaan 46 Box 2423, B-3001 Leuven, Belgium,CPMF~2 - Flemish Cluster Predictive Microbiology in Foods OPTEC - Center of Excellence Optimization in Engineering;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Predictive microbiology; Structured food model systems; Microbial colony dynamics; Microbial intracolony and intercolony interactions; Individual-based modeling; Repast Simphony;

    机译：预测微生物学;结构化食品模型系统;微生物菌落动力学;微生物菌落和菌落之间的相互作用;基于个人的建模;口吃西风;

相似文献

  • 外文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号