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首页> 外文期刊>Food microbiology >Mapping the carriage of flaA-restriction fragment length polymorphism Campylobacter genotypes on poultry carcasses through the processing chain and comparison to clinical isolates
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Mapping the carriage of flaA-restriction fragment length polymorphism Campylobacter genotypes on poultry carcasses through the processing chain and comparison to clinical isolates

机译：通过加工链在家禽屠体上绘制flaA限制性片段长度多态性弯曲杆菌基因型的运输图谱并与临床分离株进行比较

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摘要

Poultry are considered a major source for campylobacteriosis in humans. A total of 1866 Campylobacter spp. isolates collected through the poultry processing chain were typed using flaA-restriction fragment length polymorphism to measure the impact of processing on the genotypes present. Temporally related human clinical isolates (n = 497) were also typed. Isolates were obtained from whole chicken carcass rinses of chickens collected before scalding, after scalding, before immersion chilling, after immersion chilling and after packaging as well as from individual caecal samples. A total of 32 genotypes comprising at least four isolates each were recognised. Simpson's Index of Diversity (D) was calculated for each sampling site within each flock, for each flock as a whole and for the clinical isolates. From caecal collection to after packaging samples the D value did not change in two flocks, decreased in one flock and increased in the fourth flock. Dominant genotypes occurred in each flock but their constitutive percentages changed through processing. There were 23 overlapping genotypes between clinical and chicken isolates. The diversity of Campylobacter is flock dependant and may alter through processing. This study confirms that poultry are a source of campylobacteriosis in the Australian population although other sources may contribute.
机译：家禽被认为是人类弯曲菌病的主要来源。总共1866个弯曲杆菌属。使用flaA限制性片段长度多态性对通过家禽加工链收集的分离株进行分型，以测量加工过程对现有基因型的影响。还输入了与时间相关的人类临床分离株（n = 497）。从在烫伤前，烫伤后，浸入冷却前，浸入冷却后，浸入冷却后和包装后以及单独的盲肠样品中收集的鸡的全鸡car体冲洗液中获得分离物。识别出总共32种基因型，每个基因型至少包含4个分离株。计算每个鸡群内每个采样点，每个鸡群整体以及临床分离株的辛普森多样性指数（D）。从盲肠采集到包装后的样品，D值在两个鸡群中没有变化，在一个鸡群中下降，在第四只鸡群中增加。每个鸡群都有显性基因型，但其组成百分比通过加工而改变。临床分离株和鸡分离株之间有23种重叠的基因型。弯曲杆菌的多样性取决于鸡群，并可能通过加工而改变。这项研究证实，家禽是澳大利亚人群中弯曲菌病的一种来源，尽管其他来源也可能有贡献。

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  • 来源
    《Food microbiology》 |2015年第6期|116-122|共7页
  • 作者

    Lesley L. Duffy; Patrick J. Blackall; Rowland N. Cobbold; Narelle Fegan;

  • 作者单位

    CSIRO Food and Nutrition Flagship, Coopers Plains, Brisbane, Australia,The University of Queensland, School of Veterinary Science, Gatton, Australia,CSIRO Food and Nutrition Flagship, 39 Kessels Road, Coopers Plains, Brisbane 4108, Australia;

    The University of Queensland, Queensland Alliance for Agriculture and Food Innovation, Brisbane, Australia;

    The University of Queensland, School of Veterinary Science, Gatton, Australia;

    CSIRO Food and Nutrition Flagship, Werribee, Australia;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Campylobacter; Poultry; flaA-RFLP; Processing;

    机译：弯曲杆菌;家禽;flaA-RFLP;处理中;

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