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首页> 外文期刊>Food microbiology >Effect of exposure to stress conditions on propidium monoazide (PMA)-qPCR based Campylobacter enumeration in broiler carcass rinses
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Effect of exposure to stress conditions on propidium monoazide (PMA)-qPCR based Campylobacter enumeration in broiler carcass rinses

机译：暴露于应激条件对肉仔鸡漂洗液中单叠氮化丙锭（PMA）-qPCR弯曲杆菌计数的影响

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摘要

Campylobacter quantification by qPCR is unable to distinguish viable vs. dead cells in contrast to the culture-based ISO 10272-2 reference method. Propidium monoazide (PMA) has been used to overcome this disadvantage. A Campylobacter PMA-qPCR enumeration method was evaluated for its consistency and compared to the culture-based enumeration for both artificially and natural contaminated broiler carcass rinses. The PMA effect was further evaluated on stressed cells. Five conditions, commonly encountered during the slaughter process and storage (acid, heat, cold, oxidation and freezing), were inflicted to the broiler carcass rinses artificially contaminated with Campylobacter jejuni or Campylobacter coli. A better correlation between the reference method and the qPCR enumeration was obtained when PMA was used. The two cultured-based methods used showed a significant CFU reduction for heat, cold and acid stresses although the PMA-qPCR enumeration showed that viable bacteria were underestimated. Freezing showed the highest reduction effect, while the reduction extend was also overestimated by the microbiological enumeration procedure. Exposure to a mild oxidative stress was the only stress condition applied at temperatures permitting adaptation of Campylobacter and did not lead to either reduction in CFU nor in the PMA-qPCR signal.
机译：与基于培养物的ISO 10272-2参考方法相比，通过qPCR进行的弯曲杆菌定量无法区分存活细胞与死细胞。单叠氮化丙锭（PMA）已用于克服此缺点。对弯曲杆菌PMA-qPCR枚举方法的一致性进行了评估，并将其与人工和天然污染的肉鸡car体冲洗液的基于培养的枚举进行了比较。进一步评估了PMA对应激细胞的作用。宰杀过程和储存过程中经常遇到的五个条件（酸，热，冷，氧化和冷冻）对被空肠弯曲杆菌或大肠杆菌弯曲杆菌人工污染的肉鸡rinse体冲洗液造成影响。当使用PMA时，参考方法与qPCR枚举之间具有更好的相关性。尽管PMA-qPCR枚举显示活菌被低估，但使用的两种基于培养的方法在热，冷和酸胁迫下均表现出显着的CFU降低。冷冻显示出最高的还原效果，而还原范围也被微生物计数程序高估了。暴露于轻度的氧化应激是在允许弯曲杆菌适应的温度下施加的唯一应激条件，并且不会导致CFU降低或PMA-qPCR信号降低。

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  • 来源
    《Food microbiology》 |2015年第6期|182-190|共9页
  • 作者

    A. Duarte; N. Botteldoorn; W. Coucke; S. Denayer; K. Dierick; M. Uyttendaele;

  • 作者单位

    Laboratory of Food Microbiology and Food Preservation, Dept Food Safety & Food Quality Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Gent, Belgium,Institute of Public Health, Section Food-borne Pathogens, Juliette Wytsman Street 14, 1050 Brussels, Belgium,ISP-WIV, Juliette Wytsman Street 14, 1050 Brussels, Belgium;

    Institute of Public Health, Section Food-borne Pathogens, Juliette Wytsman Street 14, 1050 Brussels, Belgium;

    Institute of Public Health, Section Medical Laboratories Quality, Juliette Wytsman Street 14, 1050 Brussels, Belgium;

    Institute of Public Health, Section Food-borne Pathogens, Juliette Wytsman Street 14, 1050 Brussels, Belgium;

    Institute of Public Health, Section Food-borne Pathogens, Juliette Wytsman Street 14, 1050 Brussels, Belgium;

    Laboratory of Food Microbiology and Food Preservation, Dept Food Safety & Food Quality Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Gent, Belgium;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Propidium monoazide; Campylobacter; qPCR; Alternative method; Injured membrane / stressed cells;

    机译：单叠氮化丙锭;弯曲杆菌;定量PCR;替代方法;受损的膜/应力细胞;

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