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Location and stoichiometry of the protease CspB and the cortex-lytic enzyme SleC in Clostridium perfringens spores

机译:产气荚膜梭菌孢子中蛋白酶CspB和皮质分解酶SleC的位置和化学计量

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摘要

The protease CspB and the cortex-lytic enzyme SleC are essential for peptoglycan cortex hydrolysis during germination of spores of the Clostridium perfringens food poisoning isolate SM101. In this study, Western blot analyses were used to demonstrate that CspB and SleC are present exclusively in the C. perfringens SM101 spore coat layer fraction and absent in the lysate from decoated spores and from the purified inner spore membrane. These results indicate why decoating treatments greatly reduce both germination and apparent viability of C. perfringens spores in the absence of an exogenous lytic enzyme. In addition, quantitative Western blot analyses showed that there are approximately 2000 and 130,000 molecules of CspB and pro-SleC, respectively, per C. perfringens SM101 spore, consistent with CspB's role in acting catalytically on pro-SleC to convert this zymogen to the active enzyme.
机译:蛋白酶CspB和皮质溶解酶SleC对于产气荚膜梭状芽孢杆菌食物中毒分离物SM101的孢子萌发期间的肽聚糖皮质水解至关重要。在这项研究中,蛋白质印迹分析被用来证明CspB和SleC仅存在于产气荚膜梭菌SM101孢子涂层部分中,而从溶出的孢子和纯化的内部孢子膜的裂解物中不存在CspB和SleC。这些结果表明为什么在没有外源性分解酶的情况下,脱涂层处理会大大降低产气荚膜梭菌孢子的发芽和明显的活力。此外,定量蛋白质印迹分析表明,每个产气荚膜梭菌SM101孢子分别有大约2000和13万个CspB和pro-SleC分子,这与CspB在催化作用于pro-SleC将该酶原转化为活性酶的作用一致。酶。

著录项

  • 来源
    《Food microbiology》 |2015年第9期|83-87|共5页
  • 作者单位

    Departments of Biomedical Sciences, Oregon State University, Corvallis, OR 97331, USA,Microbiology, Oregon State University, Corvallis, OR 97331, USA,Medical Laboratories Department, College of Science Al-Zulfi, Majmaah University, Saudi Arabia;

    Department of Molecular Biology and Biophysics, University of Connecticut Health Center, Farmington, CT 06030-3305, USA;

    Departments of Biomedical Sciences, Oregon State University, Corvallis, OR 97331, USA,Gut Microbiota and Clostridia Research Group, Departamento de Ciencias Biologicas, Facultad de Ciencias Biologicas, Universidad Andres Bello, Santiago, Chile;

    Department of Molecular Biology and Biophysics, University of Connecticut Health Center, Farmington, CT 06030-3305, USA;

    Department of Molecular Biology and Biophysics, University of Connecticut Health Center, Farmington, CT 06030-3305, USA;

    Department of Molecular Biology and Biophysics, University of Connecticut Health Center, Farmington, CT 06030-3305, USA;

    Department of Biomedical Sciences, Oregon State University, 216 Dryden Hall, Corvallis, OR 97331, USA,Microbiology, Oregon State University, Corvallis, OR 97331, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Clostridium perfringens; Spores; SleC; CspB; Spore germination;

    机译:产气荚膜梭菌;孢子;SleC;CspB;孢子萌发;

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