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首页> 外文期刊>Food microbiology >Isolation and molecular characterization of Salmonella enterica serovar Enteritidis from poultry house and clinical samples during 2010
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Isolation and molecular characterization of Salmonella enterica serovar Enteritidis from poultry house and clinical samples during 2010

机译：2010年期间禽舍和临床样本中肠炎沙门氏菌肠炎沙门氏菌的分离和分子表征

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摘要

A total of 60 Salmonella enterica serovar (ser.) Enteritidis isolates, 28 from poultry houses and 32 from clinical samples, were isolated during 2010. These isolates were subjected to testing and analyzed for antibiotic resistance, virulence genes, plasmids and plasmid replicon types. To assess genetic diversity, pulsed-field gel electrophoresis (PFGE) fingerprinting, using the Xbal restriction enzyme, Multiple-Locus Variable-Number Tandem Repeat Analysis (MLVA) and plasmid profiles were performed. All isolates from poultry, and 10 out of 32 clinical isolates were sensitive to ampicillin, chloramphenicol, gentamicin, kanamycin, nalidixic acid, sulfisoxazole, streptomycin, and tetracycline. Twenty-one of thirty-two clinical isolates were resistant to ampicillin and tetracycline, and one isolate was resistant to nalidixic acid. PFGE typing of sixty ser. Enteritidis isolates by Xbal resulted in 10-12 bands and grouped into six clusters each with similarity from 95% to 81%. The MLVA analysis of sixty isolates gave 18 allele profiles with the majority of isolates displayed in three groups, and two clinical isolates found to be new in the PulseNet national MLVA database. All isolates were positive for 12 or more of the 17 virulence genes mostly found in S. enterica {spvB, spiA, pagC, msgA, invA, sipB, prgH, spaN, orgA, tolC, iroN, site, IpfC, sifA, sopB, and pefA) and negative for one gene (cdtB). All isolates carried a typical 58 kb plasmid, type Inc/FIIA. Three poultry isolates and one clinical isolate carried small plasmids with 3.8, 6, 7.6 and 11.5 kb. Ten of the clinical isolates carried plasmids, with sizes 36 and 38 kb, types IncL/M and IncN, and one isolate carried an 81 kb plasmid, type Inch Southern hybridization of a plasmid with an Inc/FIIA gene probe hybridized one large 58 kb plasmid in all isolates. Several large and small plasmids from poultry isolates were not typed by our PCR-based method. These results confirmed that PFGE fingerprinting has limited discriminatory power for ser. Enteritidis in both poultry and clinical sources. However, the plasmid and MLVA allele profiles were a useful and important epidemiology tool to discriminate outbreak strains of ser. Enteritidis from poultry and clinical samples.
机译：2010年期间共分离出60株肠炎沙门氏菌肠炎沙门氏菌（ser。）肠炎沙门氏菌分离株，其中28株来自禽舍，32份来自临床样品。这些分离株经过测试并分析了抗生素抗性，毒力基因，质粒和质粒复制子类型。为了评估遗传多样性，使用Xbal限制性内切酶，多位点可变数串联重复分析（MLVA）和质粒图谱进行了脉冲场凝胶电泳（PFGE）指纹分析。家禽的所有分离株，以及32种临床分离株中的10种对氨苄西林，氯霉素，庆大霉素，卡那霉素，萘啶酸，磺胺异恶唑，链霉素和四环素敏感。 32株临床分离株中有21株对氨苄青霉素和四环素具有抗药性，而1株分离株对萘啶酸有抗药性。 PFGE类型为60 ser。 Xbal分离的肠炎沙门氏菌产生10-12条带，并分为6个簇，每个簇的相似度从95％到81％。对60个分离株的MLVA分析得出18个等位基因概况，其中大多数分离株显示在三组中，并且在PulseNet国家MLVA数据库中发现了两个新的临床分离株。所有分离株在肠道链球菌（spvB，spiA，pagC，msgA，invA，sipB，prgH，spaN，orgA，tolC，iroN，位点，IPfC，sifA，sopB，和pefA），对一个基因（cdtB）阴性。所有分离株均携带典型的58 kb质粒，Inc / FIIA型。 3个家禽分离株和1个临床分离株带有3.8、6、7.6和11.5 kb的小质粒。十个临床分离株带有大小分别为36和38 kb的IncL / M和IncN型质粒，一个分离株带有81 kb质粒，Inch Southern型质粒与Inc / FIIA基因探针的杂交，与一个大58 kb杂交所有分离物中的质粒。通过基于PCR的方法未对禽类分离物中的几种大质粒和小质粒进行分型。这些结果证实，PFGE指纹图谱对ser的鉴别能力有限。家禽和临床来源的肠炎。但是，质粒和MLVA等位基因谱是区分ser暴发菌株的有用且重要的流行病学工具。来自家禽和临床样本的肠炎。

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  • 来源
    《Food microbiology》 |2014年第4期|67-74|共8页
  • 作者

    Ezat H. Mezal; Ashley Sabol; Mariam A. Khan; Nawab Ali; Rossina Stefanova; Ashraf A. Khan;

  • 作者单位

    Microbiology Division, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR 72079, USA,University of Arkansas at Little Rock, Little Rock, AR 72205, USA University of Thi-Qar, Science of College, Biology of Dept., Thi-Qar, Iraq;

    Centers for Disease Control and Prevention, Atlanta, GA 30333, USA;

    University of Central Arkansas, Conway, AR, USA;

    University of Arkansas at Little Rock, Little Rock, AR 72205, USA;

    The Arkansas Department of Health, Little Rock, AR, USA;

    Microbiology Division, National Center for Toxicological Research, US. Food and Drug Administration, Jefferson, AR 72079, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Salmonella Enteritidis; MLVA; Pulsed-field gel electrophoresis; Plasmid;

    机译：肠炎沙门氏菌MLVA;脉冲场凝胶电泳;质粒;

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