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Cultivable microorganisms associated with honeys of different geographical and botanical origin

机译：与不同地理和植物来源的蜂蜜相关的可培养微生物

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摘要

In this study, the composition of the cultivable microbial populations of 38 nectar honey and honeydew honey samples of different botanical and geographical origin were assessed. After growth in specific media, various colonies with different appearance were isolated and purified before phenotypic (morphological, physiological and biochemical traits) and genotypic [randomly amplified polymorphic DNA (RAPD), repetitive DNA elements-PCR (rep-PCR) and restriction fragment length polymorphism (RFLP)] differentiation. The identification was carried out by 16S rRNA gene sequencing for bacteria and, in addition to RFLP, by sequencing the D1/D2 region of the 26S rRNA gene for yeasts and the 5.8S-ITS rRNA region for filamentous fungi. The results showed the presence of 13 species of bacteria, 5 of yeasts and 17 of filamentous fungi; the species most frequently isolated were Bacillus amyloliquefaciens, Zygosaccharomyces mellis and Aspergillus niger for the three microbial groups, respectively. The highest microbial diversity was found in multifloral honeys. No correlation among the microbial species and the botanical/geographical origin was found, but some strains were highly adapted to these matrices since they were found in several samples of different origin.
机译：在这项研究中，评估了38种花蜜和不同植物和地理来源的蜜露蜂蜜样品的可培养微生物种群组成。在特定培养基中生长后，在表型（形态，生理和生化特性）和基因型（随机扩增多态性DNA（RAPD），重复性DNA元素-PCR（rep-PCR）和限制性片段长度）之前，分离并纯化了具有不同外观的各种菌落。多态性（RFLP）]分化。通过对细菌的16S rRNA基因测序以及除RFLP之外的测序，对26S rRNA基因的D1 / D2区（对酵母）和5.8S-ITS rRNA区（对丝状真菌）进行测序。结果显示存在13种细菌，5种酵母和17种丝状真菌。三个微生物组中分离最频繁的物种分别是解淀粉芽孢杆菌，梅氏酵母和黑曲霉。在多花蜂蜜中发现了最高的微生物多样性。没有发现微生物种类与植物/地理起源之间的相关性，但由于在不同来源的几个样品中发现了某些菌株，因此它们对这些基质具有高度适应性。

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  • 来源
    《Food microbiology》 |2014年第4期|284-294|共11页
  • 作者

    Milko Sinacori; Nicola Francesca; Antonio Alfonzo; Margherita Cruciata; Ciro Sannino; Luca Settanni; Giancarlo Moschetti;

  • 作者单位

    Department of Agricultural and Forestry Science, University of Palermo, Viale delle Scienze 4, 90128 Palermo, Italy;

    Department of Agricultural and Forestry Science, University of Palermo, Viale delle Scienze 4, 90128 Palermo, Italy;

    Department of Agricultural and Forestry Science, University of Palermo, Viale delle Scienze 4, 90128 Palermo, Italy;

    Department of Agricultural and Forestry Science, University of Palermo, Viale delle Scienze 4, 90128 Palermo, Italy;

    Department of Agricultural and Forestry Science, University of Palermo, Viale delle Scienze 4, 90128 Palermo, Italy;

    Department of Agricultural and Forestry Science, University of Palermo, Viale delle Scienze 4, 90128 Palermo, Italy;

    Department of Agricultural and Forestry Science, University of Palermo, Viale delle Scienze 4, 90128 Palermo, Italy;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Bacteria; Culture-dependent approach; Filamentous fungi; Honey; Molecular characterisation; Yeasts;

    机译：菌;依赖文化的方法;丝状真菌;蜜糖;分子表征;酵母;

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