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首页> 外文期刊>Food microbiology >Monitoring the succession of the biota grown on a selective medium for pseudomonads during storage of minced beef with molecular-based methods
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Monitoring the succession of the biota grown on a selective medium for pseudomonads during storage of minced beef with molecular-based methods

机译：使用基于分子的方法监测切碎的牛肉在储存过程中在假单胞菌选择性培养基上生长的生物群的演替

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摘要

In the present study, the succession of the biota grown on a selective medium for pseudomonads (pseudomonas agar based medium - PAB) during the storage of meat under different conditions was monitored. Thus, minced beef was stored aerobically and under modified atmosphere packaging in the presence (MAP+) and absence (MAP-) of oregano essential oil at 0, 5,10 and 15 ℃. A total of 267 pure cultures were recovered from PAB throughout the storage period and subjected to PCR-Denaturing gradient gel electrophoresis (PCR-DCGE) for their differentiation. In parallel, the direct analysis of the whole cultivable community (WCC) from the same medium was applied. These two approaches were used in order to indicate the lack of selectivity. Fifteen different DGGE fingerprints were obtained after PCR - DGGE analysis of the isolates, which were assigned to Pseudomonas putida (3 fingerprints), Pseudomonas fragi and Pseudomonas fluorescens, Pseudomonas spp., Serratia liquefaciens (2), Citrobacter freundii, Serratia grimesii, Hafnia alvei (3), Rahnella spp. and Morganella morganii. Twelve of them occurred during the direct analysis of the WCC. The biota succession found to be affected from the different storage conditions. However, the outcome of the two strategies was quite different, which is leading to the use of different appropriated molecular approaches in order to widen the knowledge of bacterial succession of meat.
机译：在本研究中，监测了在不同条件下肉类存储期间在假单胞菌选择性培养基（假单胞菌琼脂基培养基-PAB）上生长的生物群的连续性。因此，切碎的牛肉在0、5、10和15℃下在牛至精油存在（MAP +）和不存在（MAP-）的情况下，需氧包装并在气调包装下存储。在整个存储期间，共从PAB中回收了267种纯培养物，并进行了PCR变性梯度凝胶电泳（PCR-DCGE）进行分化。同时，对来自同一培养基的整个可耕种群落（WCC）进行了直接分析。使用这两种方法是为了表明缺乏选择性。经PCR-DGGE分析后，分离物获得了15种不同的DGGE指纹，分别为恶臭假单胞菌（3个指纹），脆弱假单胞菌和荧光假单胞菌，假单胞菌，液化粘质沙雷氏菌（2），弗氏柠檬酸杆菌，褐腐沙雷氏菌（3），Rahnella spp。和摩根氏菌在WCC的直接分析中，有十二起发生。发现不同的储存条件会影响生物群落的演替。但是，这两种策略的结果截然不同，这导致使用不同的适当分子方法来拓宽肉类细菌继承的知识。

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  • 来源
    《Food microbiology》 |2013年第1期|62-69|共8页
  • 作者

    Agapi I. Doulgeraki; George-John E. Nychas;

  • 作者单位

    Laboratory of Food Microbiology and Biotechnology, Department of Food Science and Technology, Agricultural University of Athens, lera Odos 75, Athens 11855, Greece;

    Laboratory of Food Microbiology and Biotechnology, Department of Food Science and Technology, Agricultural University of Athens, lera Odos 75, Athens 11855, Greece;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    meat; pseudomonas; enterobacteria; lack of selectivity; pcr-dgge; pfge;

    机译：肉;假单胞菌肠杆菌;缺乏选择性;pcr-dgge;pfge;

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