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Attachment behaviour of Escherichia coli K12 and Salmonella Typhimurium P6 on food contact surfaces for food transportation

机译:大肠埃希菌K12和鼠伤寒沙门氏菌P6在食物接触表面上的附着行为

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摘要

The role of cargo container lining materials aluminium, a fibre reinforced plastic (FRP) and stainless steel in bacterial cross contamination during transport was assessed. For this, attachment and detachment of Escherichia coli K12 and Salmonella Typhimurium P6 on the three surfaces in the absence or presence of residues were evaluated. Observations were correlated with water contact angles of the materials (hydrophobicity) and roughness profile (R_a). Attachment of the organisms was negatively correlated to the hydrophobicity of the three materials with r = -0.869 and -0.861 for E. coli K12 and S. Typhimurium P6 respectively. Correlation with roughness average was poor; r = -0.425 and -0.413 respectively for £. coli K12 and S. Typhimurium P6. Presence of residue caused significant reduction (p < 0.05) in the levels of bacteria attached to all materials, but made attached bacteria significantly more difficult to detach by either of two rinsing systems from all three surfaces. Explanation for these observations could be made in part from scanning electron micrographs which showed significantly more bacteria sitting on patches of residue when it was introduced to the surfaces, compared to the bare material sections of the same surfaces. We report these observations for the first time for aluminium and the FRP material and in part for stainless steel. The S. Typhimurium P6 strain also had significantly higher level of attachment than the E. coli K12 strain. Our findings show that food residue and soils affect the extent and amount of bacteria attaching to abiotic surfaces by altering the surface contact properties for the bacteria. Physi-cochemical properties like hydrophobicity appear to be a better basis for material selection for hygienic design of containers, than the traditional use of R_a.
机译:评估了货物集装箱内衬材料铝,纤维增强塑料(FRP)和不锈钢在运输过程中细菌交叉污染中的作用。为此,评估了在没有或没有残留物的情况下,大肠杆菌K12和鼠伤寒沙门氏菌P6在三个表面上的附着和分离。观察结果与材料的水接触角(疏水性)和粗糙度轮廓(R_a)相关。生物体的附着与三种物质的疏水性呈负相关,对于大肠杆菌K12和鼠伤寒沙门氏菌P6,r = -0.869和-0.861。与粗糙度平均值的相关性较差; r分别为r = -0.425和-0.413。大肠杆菌K12和鼠伤寒沙门氏菌P6。残留物的存在会导致附着在所有材料上的细菌水平显着降低(p <0.05),但是使附着的细菌更难以通过两种冲洗系统从所有三个表面上分离。这些观察结果的部分解释可能是由扫描电子显微照片得出的,与相同表面的裸露材料部分相比,扫描电子显微镜照片显示,当残留物被引入表面时,残留在其上的细菌明显更多。我们首次报告了铝和FRP材料的观察结果,部分报告了不锈钢的观察结果。鼠伤寒沙门氏菌P6菌株的附着水平也比大肠杆菌K12菌株高得多。我们的发现表明,食物残渣和土壤会通过改变细菌的表面接触特性来影响附着在非生物表面上的细菌的程度和数量。与传统的R_a相比,像疏水性这样的物理化学特性似乎是容器卫生设计中选择材料的更好基础。

著录项

  • 来源
    《Food microbiology》 |2012年第2期|p.139-147|共9页
  • 作者单位

    Department of Food Science, Centre for Advanced Food Studies (LMC), Faculty of Life Sciences, University of Copenhagen, Rolighedsvej 30, 4, DK-1958 Frederiksberg C, Denmark;

    Department of Food Science, Centre for Advanced Food Studies (LMC), Faculty of Life Sciences, University of Copenhagen, Rolighedsvej 30, 4, DK-1958 Frederiksberg C, Denmark;

    Department of Food Science, Centre for Advanced Food Studies (LMC), Faculty of Life Sciences, University of Copenhagen, Rolighedsvej 30, 4, DK-1958 Frederiksberg C, Denmark;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    attachment; transportation; hygienic design; cross contamination; hydrophobicity; roughness;

    机译:附件;运输;卫生设计;交叉污染;疏水性;粗糙度;

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