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Inhibition of Listeria monocytogenes by Enterococcus mundtii isolated from soil

机译：从土壤中分离的芒氏肠球菌对单核细胞增生李斯特菌的抑制作用

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摘要

Two bacterial isolates with inhibitory activity against Listeria monocytogenes and Enterococcus faecalis were obtained from soil. Genotypic and phenotypic characterization identified them as Enterococcus mundtii, a species whose ability to compete with L monocytogenes is relatively unexplored compared to other members of the genus. The thermal stability of the inhibitory factor and its sensitivity to proteolytic enzymes indicate that it is most likely a bacteriocin. Both isolates grew at comparable rates to L. monocytogenes at 5 ℃ and 10 ℃ in vitro. One isolate killed L monocytogenes when it reached concentrations of 10~6-10~8 CFU ml~(-1). Minimum inocula of 10~6 and 10~5 CFU ml~(-1) of £ mundtii were required to reduce and maintain L. monocytogenes concentrations beneath the level of detection at 5 ℃ and 10 ℃, respectively. In situ experiments at 5 ℃ showed that E. mundtii inhibited the growth of L. monocytogenes on vacuum-packed cold smoked salmon during its four week shelf life. E. mundtii could, therefore, control the growth of L. monocytogenes at low temperatures, indicating a potential application in controlling this pathogen in chilled foods. To control growth of Listeria, the concentration of E. mundtii needs to be high, but it is possible that a purified bacteriocin could be used to achieve the same effect.
机译：从土壤中获得了两种对单核细胞增生李斯特菌和粪肠球菌具有抑制活性的细菌。基因型和表型鉴定将它们鉴定为芒球菌，与该属其他成员相比，与单核细胞增生李斯特菌竞争的能力相对较弱。抑制因子的热稳定性及其对蛋白水解酶的敏感性表明，它很可能是细菌素。两种分离物在体外5℃和10℃下均以与单核细胞增生李斯特菌相当的速率生长。一种分离株在浓度达到10〜6-10〜8 CFU ml〜（-1）时杀死单核细胞增生李斯特菌。为了降低和维持单核细胞增生李斯特氏菌的浓度分别低于5℃和10℃的检测水平，最低接种量为10〜6 CFU ml〜（-1）£mundtii。在5℃的原位实验表明，蒙地芽孢杆菌在真空包装的冷熏鲑鱼的保质期为4周时抑制了单核细胞增生李斯特氏菌的生长。因此，芒氏大肠杆菌可以在低温下控制单核细胞增生李斯特菌的生长，这表明在控制冷藏食品中这种病原体方面有潜在的应用。为了控制利斯特氏菌的生长，需要提高芒氏大肠杆菌的浓度，但是有可能可以使用纯化的细菌素来达到同样的效果。

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  • 来源
    《Food microbiology》 |2012年第2期|p.354-360|共7页
  • 作者

    T. Bigwood; J.A. Hudson; J. Cooney; L Mclntyre; C. Billington; J.A. Heinemann; F. Wall;

  • 作者单位

    Food Safety Programme, Institute of Environmental Science and Research Ltd (ESR), Christchurch Science Centre, PO Box 29-181, Ham, Christchurch, New Zealand ,School of Biological Sciences, University of Canterbury, Christchurch, NewZealand;

    Food Safety Programme, Institute of Environmental Science and Research Ltd (ESR), Christchurch Science Centre, PO Box 29-181, Ham, Christchurch, New Zealand ,Department of Food Science and Agri-Food Supply Management, Harper Adams University College, Edgmond, Newport, Shropshire, United Kingdom.;

    Food Safety Programme, Institute of Environmental Science and Research Ltd (ESR), Christchurch Science Centre, PO Box 29-181, Ham, Christchurch, New Zealand ,CPIT, 130 Madras Street, Christchurch, New Zealand;

    Food Safety Programme, Institute of Environmental Science and Research Ltd (ESR), Christchurch Science Centre, PO Box 29-181, Ham, Christchurch, New Zealand;

    Food Safety Programme, Institute of Environmental Science and Research Ltd (ESR), Christchurch Science Centre, PO Box 29-181, Ham, Christchurch, New Zealand;

    School of Biological Sciences, University of Canterbury, Christchurch, NewZealand;

    CPIT, 130 Madras Street, Christchurch, New Zealand;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    enterococcus mundtii; biocontrol; listeria monocytogenes; food; bacteriocin;

    机译：普通肠球菌;生物防治;单核细胞增生李斯特菌;食品;细菌素;

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