首页> 外文期刊>Food microbiology >Inactivation of hepatitis A virus and norovirus surrogate in suspension and on food-contact surfaces using pulsed UV light (pulsed light inactivation of food-borne viruses)
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Inactivation of hepatitis A virus and norovirus surrogate in suspension and on food-contact surfaces using pulsed UV light (pulsed light inactivation of food-borne viruses)

机译:甲型肝炎病毒和诺如病毒的灭活在悬浮液中和与食物接触的表面上使用脉冲紫外光替代(食源病毒的脉冲光灭活)

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摘要

This study was conducted to evaluate the inactivation of murine norovirus (MNV-1) and hepatitis A virus (HAV) by pulsed ultraviolet (UV) light. MNV-1 was used as a model for human norovirus. Viral suspensions of about 10~6 PFU/ml were exposed to pulses of UV light for different times and at different distances in a Xenon Steripulse device (model RS-3000C). Inactivation studies were also carried out on 1-cm~2 stainless steel and polyvinyl chloride disks with 10~5 PFU/ml. Inactivation of MNV-1 and HAV at 10.5 cm from the UV source was greater on inert surfaces than in suspension. The presence of organic matter (fetal bovine serum) reduced the effectiveness of pulsed light both in suspension and on surfaces. However, 2-s treatment in the absence of FBS completely inactivated (5 log reduction) the viral load at different distances tested, whether in suspension (MNV-1) or on disks (MNV-1 and HAV). The same treatment in the presence of fetal bovine serum (5%) allowed a reduction of about 3 log. This study showed that short duration pulses represent an excellent alternative for inactivation of food-borne viruses. This technology could be used to inactivate viruses in drinking water or on food-handling surfaces.
机译:进行这项研究以评估脉冲紫外线(UV)对小鼠诺如病毒(MNV-1)和甲型肝炎病毒(HAV)的灭活作用。 MNV-1被用作人类诺如病毒的模型。在Xenon Steripulse装置(型号RS-3000C)中,将约10〜6 PFU / ml的病毒悬浮液暴露于紫外线脉冲中不同的时间和不同的距离。灭活研究还以10〜5 PFU / ml在1-cm〜2不锈钢和聚氯乙烯盘上进行。在惰性表面上,与悬浮液相比,MNV-1和HAV在距紫外线源10.5 cm处的失活更大。有机物(胎牛血清)的存在降低了悬浮液和表面上脉冲光的有效性。但是,在没有FBS的情况下进行2-s处理,无论是悬液(MNV-1)还是盘片(MNV-1和HAV),在不同的测试距离下都完全灭活了病毒负荷(减少了5 log)。在胎牛血清(5%)存在下的相同处理允许减少约3log。这项研究表明,短时脉冲是灭活食源性病毒的绝佳选择。该技术可用于灭活饮用水或食品处理表面中的病毒。

著录项

  • 来源
    《Food microbiology》 |2011年第3期|p.568-572|共5页
  • 作者单位

    Institut des Nutraceutiques et des Aliments Fonctionnels, Universite Laval, Quebec, Quebec C1K 7P4, Canada;

    Institut des Nutraceutiques et des Aliments Fonctionnels, Universite Laval, Quebec, Quebec C1K 7P4, Canada;

    Institut des Nutraceutiques et des Aliments Fonctionnels, Universite Laval, Quebec, Quebec C1K 7P4, Canada;

    Institut des Nutraceutiques et des Aliments Fonctionnels, Universite Laval, Quebec, Quebec C1K 7P4, Canada;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    MNV; HAV; pulsed UV light; virus inactivation; food-contact surfaces;

    机译:MNV;甲型肝炎脉冲紫外线病毒灭活;食物接触面;

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