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Characterization of the yeast ecosystem in grape must and wine using real-time PCR

机译：使用实时PCR表征葡萄汁和葡萄酒中的酵母生态系统

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摘要

The complex microbial ecosystem of grape must and wine harbours a wide diversity of yeast species. Specific oligonucleotide primers for real-time quantitative PCR(QPCR) were designed to analyse several important non-Saccharomyces yeasts (Issatchenkia orientalis, Metschnikowia pulcherrima, Torulaspora delbrueckii, Candida zemplinina and Hanseniaspora spp.) and Saccharomyces spp. in fresh wine must, during fermentation and in the finished wine. The specificity of all primer couples for their target yeast species were validated and the QPCR methods developed were compared with a classic approach of colony identification by RFLP-ITS-PCR on cultured samples. Once the methods had been developed and validated, they were used to study these non-Saccharomyces yeasts in wine samples and to monitor their dynamics throughout the fermentation process. This study confirms the usefulness and the relevance of QPCR for studying non-Saccharomyces yeasts in the complex yeast ecosystem of grape must and wine.
机译：葡萄汁和葡萄酒的复杂微生物生态系统蕴藏着多种多样的酵母菌。设计了用于实时定量PCR（QPCR）的特定寡核苷酸引物，以分析几种重要的非酿酒酵母酵母（东方伊萨酵母，Metschnikowia pulcherrima，德鲁拉酵母，德氏假丝酵母和汉森酵母属）和酿酒酵母。必须在新鲜葡萄酒中，发酵期间和成品葡萄酒中使用。验证了所有引物对对其目标酵母菌种的特异性，并将开发的QPCR方法与通过RFLP-ITS-PCR对培养样品进行菌落鉴定的经典方法进行了比较。一旦开发并验证了这些方法，就将它们用于研究葡萄酒样品中的这些非酿酒酵母，并在整个发酵过程中监测其动态。这项研究证实了QPCR在研究葡萄汁和葡萄酒的复杂酵母生态系统中研究非酿酒酵母的有用性和相关性。

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  • 来源
    《Food microbiology》 |2010年第5期|559-567|共9页
  • 作者

    K. Zott; O. Claisse; P. Lucas; J. Coulon; A. Lonvaud-Funel; I. Masneuf-Pomarede;

  • 作者单位

    Universite de Bordeaux, ISVV, Bordeaux Aquitaine, INRA UMR 1219, 210 Chemin de Leysotte, CS 50008, 33882 Villenave d'Ornon Cedex, France ENITA de Bordeaux, Cours du General de Gaulle, CS 40201, 33175 Gradignan Cedex, France;

    Universite de Bordeaux, ISVV, Bordeaux Aquitaine, INRA UMR 1219, 210 Chemin de Leysotte, CS 50008, 33882 Villenave d'Ornon Cedex, France;

    Universite de Bordeaux, ISVV, Bordeaux Aquitaine, INRA UMR 1219, 210 Chemin de Leysotte, CS 50008, 33882 Villenave d'Ornon Cedex, France;

    Microflora, ISVV, Bordeaux Aquitaine, INRA UMR 1219, 210 Chemin de Leysotte, CS 50008, 33882 Villenave d'Ornon Cedex, France;

    Universite de Bordeaux, ISVV, Bordeaux Aquitaine, INRA UMR 1219, 210 Chemin de Leysotte, CS 50008, 33882 Villenave d'Ornon Cedex, France;

    Universite de Bordeaux, ISVV, Bordeaux Aquitaine, INRA UMR 1219, 210 Chemin de Leysotte, CS 50008, 33882 Villenave d'Ornon Cedex, France ENITA de Bordeaux, Cours du General de Gaulle, CS 40201, 33175 Gradignan Cedex, France;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    real-time PCR; non-saccharomyces yeasts ecology; grape must; wine;

    机译：实时PCR;非酵母菌生态学;葡萄必须葡萄酒;

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