• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Food microbiology >Prevalence and contamination patterns of Listeria monocytogenes in catfish processing environment and fresh fillets
【24h】

Prevalence and contamination patterns of Listeria monocytogenes in catfish processing environment and fresh fillets

机译：cat鱼加工环境和新鲜鱼片中李斯特菌李斯特菌的流行和污染模式

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Catfish skins, intestines, fresh fillets, processing surfaces at different production stages, chiller water and non-food contact surfaces were sampled for Listeria monocytogenes and other Listeria species. Among 315 samples, prevalence of L. monocytogenes, Listeria innocua and a group of Listeria seeligeri-Listeria welshimeri-Listeria ivanovii was 21.6, 13.0 and 29.5%, respectively. No Listeria grayi was detected in this survey. While no L. monocytogenes strains were isolated from catfish skins and intestines, the strains were found with a frequency of 76.7% in chilled fresh catfish fillets and 43.3% in unchilled fillets. L. monocytogenes and Listeria spp. were also detected in fish contact surfaces such as deheading machine, trimming board, chiller water, conveyor belts at different stages, and fillet weighing table. Among L. monocytogenes, 1/2b (47.0%), 3b (16.0%) and 4c (14%) were the predominant serotypes isolated, whereas 4b, 4e, 1/2c and 1/2a were detected at much lower frequencies. Genotype analyses of L. monocytogenes isolates using serotyping, pulsed-field gel electrophoresis (PFGE) and enterobacterial repetitive intergenic consensus (ERIC)-PCR revealed that chiller water represented an important contamination source of L. monocytogenes in the chilled catfish fillets of two processing facilities, whereas fillet weighing table significantly contributed to the catfish fillet contamination of the third facility. This study suggests that L. monocytogenes contamination in the processed catfish fillets originates from the processing environment, rather than directly from catfish. Results from this study can aid the catfish industry to develop a plant-specific proper cleaning and sanitation procedure for equipment and the processing environment designed to specifically target L. monocytogenes contamination.
机译：对mono鱼皮，肠，新鲜鱼片，不同生产阶段的加工表面，冷水和非食品接触表面进行了抽样，以检测李斯特菌和其他李斯特菌。在315个样本中，单核细胞增生李斯特菌，无病李斯特菌和一组西氏李斯特菌-welshimeri-伊斯特氏菌李斯特菌的患病率分别为21.6、13.0和29.5％。在该调查中未检测到灰色李斯特菌。虽然没有从fish鱼皮和肠中分离出单核细胞增生李斯特氏菌菌株，但在冷藏的新鲜cat鱼鱼片中发现这些菌株的频率为76.7％，而在未冷藏的鱼片中发现为43.3％。单核细胞增生李斯特菌和李斯特菌属。还可以在去鱼头机，修剪板，冷却水，不同阶段的传送带和鱼片称重台等鱼接触面中检测到。在单核细胞增生李斯特氏菌中，分离出的主要血清型为1 / 2b（47.0％），3b（16.0％）和4c（14％），而在低得多的频率下检测到4b，4e，1 / 2c和1 / 2a。使用血清分型，脉冲场凝胶电泳（PFGE）和肠细菌重复性基因间共识（ERIC）-PCR对单核细胞增生李斯特菌分离株进行基因型分析，结果表明，冷水代表了两个加工设施的冷冻cat鱼鱼片中单核细胞增生李斯特菌的重要污染源。 ，而鱼片重量表则是造成第三家工厂the鱼鱼片污染的重要原因。这项研究表明，加工cat鱼鱼片中的单核细胞增生李斯特氏菌污染源自加工环境，而不是直接来自from鱼。这项研究的结果可以帮助the鱼业开发针对设备和加工环境的，针对植物的适当清洁和卫生程序，旨在专门针对单核细胞增生李斯特菌污染。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号