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首页> 外文期刊>Food microbiology >Molecular Methods To Evaluate Biodiversity In Bacillus Cereus And Bacillus Thuringiensis Strains From Different Origins
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Molecular Methods To Evaluate Biodiversity In Bacillus Cereus And Bacillus Thuringiensis Strains From Different Origins

机译：评价不同来源的蜡状芽孢杆菌和苏云金芽孢杆菌菌株生物多样性的分子方法

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摘要

The spore-forming genus Bacillus includes species of-industrial, clinical and environmental significance. The possibility of differentiating between Bacillus cereus and Bacillus thuringiensis, toxin producers associated with illness, is a real need in monitoring potentially contaminated foods to understand the real distribution of B. cereus/B. thuringiensis in different outbreak cases. As the use of DNA comparison obtains clearer results than classical microbiological methods in distinguishing B. cereus from B. thuringiensis in this work PCR-TTGE (Temporal Temperature Gradient gel Electrophoresis), rep-PCR and RAPD-PCR methods have been compared to assess the intra- and inter-specific variability of B. cereus and B. thuringiensis. 80 strains of B. cereus and B. thuringiensis isolated from food, patients and pesticides were analyzed using a gyrB gene DNA sequence in TTGE; primer M13 in the RAPD-PCR and primers REPlDTand REP2DT in the rep-PCR methods. A widespread distribution of the electrophoretic profiles was obtained either for B. cereus or for B. thuringiensis using TTGE. rep-PCR and RAPD-PCR were not always able to group strains from the same origin or belonging to the same species. The fingerprints obtained with the rep- and RAPD-PCR methods confirm the high intraspecific variability present in B. cereus and B. thuringiensis indicating the difficulty to discriminate between these two species in outbreak cases.
机译：形成芽孢的芽孢杆菌属包括工业，临床和环境意义的种。区分与疾病相关的毒素生产者蜡状芽孢杆菌和苏云金芽孢杆菌的可能性，是真正需要监控潜在污染的食品以了解蜡状芽孢杆菌/ B的真实分布。苏云金芽孢杆菌在不同的暴发病例中。在这项工作中，使用DNA比较在区分苏云金芽孢杆菌和苏云金芽孢杆菌方面获得了比传统微生物学方法更清晰的结果PCR-TTGE（温度梯度凝胶电泳），因此比较了rep-PCR和RAPD-PCR方法来评估蜡状芽孢杆菌和苏云金芽孢杆菌的种内和种间变异性。使用TTGE中的gyrB基因DNA序列分析了从食品，患者和农药中分离的80株蜡状芽孢杆菌和苏云金芽孢杆菌。 RAPD-PCR中的引物M13和rep-PCR方法中的引物REP1DT和REP2DT。蜡状芽孢杆菌或苏云金芽孢杆菌使用TTGE获得了广泛的电泳图谱分布。 rep-PCR和RAPD-PCR并不总是能够对来自相同起源或属于同一物种的菌株进行分组。用rep和RAPD-PCR方法获得的指纹图谱证实了蜡状芽孢杆菌和苏云金芽孢杆菌中存在较高的种内变异性，这表明在暴发病例中很难区分这两个物种。

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