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首页> 外文期刊>Food microbiology >Characterization of molds isolated from smoked paprika by PCR-RFLP and micellar electrokinetic capillary electrophoresis
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Characterization of molds isolated from smoked paprika by PCR-RFLP and micellar electrokinetic capillary electrophoresis

机译：通过PCR-RFLP和胶束电动毛细管电泳鉴定从熏制辣椒粉中分离的霉菌

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摘要

Molds are common contaminants of paprika meat products. The drying and storage stages of paprika processing are critical because they can provide molds with the conditions particularly appropriate for their growth and proliferation. Thus, an efficient and accurate characterization of the toxigenic molds of paprika is necessary. An RFLP analysis of the rRNA genes was performed by using a Taql restriction enzyme. In addition, a micellar electrokinetic capillary chromatography (MECC) method was tested to analyze secondary metabolites produced by mold strains commonly found in paprika. This study was confirmed with a 5.8S-ITS region sequence analysis. A total of 31 isolates were identified by RFLP and MECC analysis. These showed stable RFLP profiles that were clearly different for the different genera and species, and were grouped into clusters together with the profiles of the 16 reference strains. MECC analysis provided additional characteristic peak patterns for the characterization of the mold species present. The characterized isolates were species of the genera Fusarium spp., Aspergillus spp., Penicillium spp., Cladosporium spp., Mucor spp. and Phlebia spp. The identifications were confirmed by the 5.8S-ITS region sequence analysis and by a BLAST search of the GenBank database. RFLP patterns with Taql restriction enzyme and MECC profiles, either singly or combined, could be of great interest to distinguish molds in paprika.
机译：霉菌是辣椒粉肉制品的常见污染物。辣椒粉加工的干燥和储存阶段至关重要，因为它们可以为霉菌提供特别适合其生长和繁殖的条件。因此，有必要对辣椒粉的产毒霉菌进行有效而准确的表征。通过使用Taql限制酶对rRNA基因进行RFLP分析。另外，测试了胶束电动毛细管色谱法（MECC）以分析由辣椒粉中常见的霉菌菌株产生的次生代谢产物。 5.8S-ITS区域序列分析证实了这项研究。通过RFLP和MECC分析鉴定出总共31个分离株。这些显示稳定的RFLP谱图，对于不同的属和物种，RFLP谱图明显不同，并且与16个参考菌株的谱图一起归为一类。 MECC分析为表征存在的霉菌提供了额外的特征峰模式。表征的分离物是镰刀菌属，曲霉属，青霉属，枝孢属，毛霉属的种。和Phlebia spp。通过5.8S-ITS区域序列分析和GenBank数据库的BLAST搜索来确认鉴定。带有Taql限制酶和MECC分布图的RFLP模式（单独或组合）可能对区分辣椒粉中的霉菌非常有用。

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  • 来源
    《Food microbiology》 |2009年第8期|776-782|共7页
  • 作者

    Santiago Ruiz-Moyano; Maria J. Benito; Alberto Martin; Emilio Aranda; Alejandro Hernandez; Maria G. Cordoba;

  • 作者单位

    Nutrition y Bromatologia, Escuela de Ingenierias Agrarias, Universidad de Extremadura, Ctra. de Caceres s, 06071 Badajoz, Spain;

    Nutrition y Bromatologia, Escuela de Ingenierias Agrarias, Universidad de Extremadura, Ctra. de Caceres s, 06071 Badajoz, Spain;

    Nutrition y Bromatologia, Escuela de Ingenierias Agrarias, Universidad de Extremadura, Ctra. de Caceres s, 06071 Badajoz, Spain;

    Nutrition y Bromatologia, Escuela de Ingenierias Agrarias, Universidad de Extremadura, Ctra. de Caceres s, 06071 Badajoz, Spain;

    Nutrition y Bromatologia, Escuela de Ingenierias Agrarias, Universidad de Extremadura, Ctra. de Caceres s, 06071 Badajoz, Spain;

    Nutrition y Bromatologia, Escuela de Ingenierias Agrarias, Universidad de Extremadura, Ctra. de Caceres s, 06071 Badajoz, Spain;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    molds; PCR-RFLP; ITS; paprika; genotype;

    机译：模具PCR-RFLP;它的;辣椒;基因型;

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