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Microbial diversity changes in soybean sprouts treated with enterocin AS-48

机译：肠素AS-48处理的大豆芽中微生物多样性的变化

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摘要

Seed sprouts may act as vehicles for foodborne pathogenic bacteria. In the present study, the effect of washing treatment with the enterococcal bacteriocin enterocin AS-48 on the microbiota of two batches of soybean sprouts was studied by culture-dependent and independent methods throughout storage at 10 ℃. Viable cell counts of bacteriocin-treated samples revealed some modifications only for lactic acid bacteria and enterococci during storage. In the control samples from batch 1, the culture-independent DGGE analysis revealed species from genera Rahnella and Serratia as the predominant bacteria at early stages. Several bands corresponding to other genera (two Pantoea bands, one Escherichia band, and five Enterobacter bands) were also detected during storage of control samples, especially at days 3 and 5, while one Rahnella band disappeared. By contrast, some of the enterobacteria (Pantoea Escherichia and Enterobacter) were not detected or showed very faint bands in batch 1 bacteriocin-treated samples, in which two new and intense bands corresponding to genera Enterococcus and Leuconostoc were detected. Batch 2 showed a more homogeneous bacterial population, composed mainly by species of genus Enterobacter together with Pantoea. The major modifications detected in the bacteriocin-treated samples from batch 2 included the loss of one genus Enterobacter band at days 3, 5 and 7, and the detection of a new band corresponding to genus Leuconostoc at days 5 and 7. These results suggest that bacteriocin treatment disturbs the microbia! balance in sprouts, producing changes in the microbial profile that cannot be detected by culture-dependent methods. The results also encourage the use of culture-independent methods to gain more insights into the global effects of bacteriocins in food systems.
机译：种子芽可作为食源性致病细菌的媒介。在本研究中，通过培养依赖性和独立性方法研究了在10℃下整个贮藏过程中肠球菌细菌肠球菌肠素AS-48洗涤处理对两批大豆豆芽微生物群的影响。经细菌素处理的样品的活细胞计数显示，仅在储存期间对乳酸菌和肠球菌有一些修饰。在第1批的对照样品中，不依赖培养物的DGGE分析显示，早期阶段的主要细菌是Rahnella和Serratia属。在对照样品保存期间，特别是在第3天和第5天，还检测到与其他属相对应的几个条带（两个Pantoea乐队，一个Escherichia乐队和五个Enterobacter乐队），而一个Rahnella乐队消失了。相比之下，在第1批细菌素处理的样品中，未检测到某些肠杆菌（大肠埃希氏菌和肠杆菌）或显示出非常微弱的条带，其中检测到了两个新的强条带，分别对应于肠球菌和亮粘菌属。第2批显示更均匀的细菌种群，主要由肠杆菌属和泛菌组成。在批次2的经细菌素处理的样品中检测到的主要修饰包括在第3、5和7天失去了一个肠杆菌属条带，并在第5和7天检测到了一个与Leuconostoc属相对应的新谱带。这些结果表明，细菌素治疗会干扰微生物！平衡新芽，导致微生物特征发生变化，而依赖于培养的方法则无法检测到这种变化。结果还鼓励使用非培养依赖性方法，以更深入地了解细菌素在食品系统中的整体作用。

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  • 来源
    《Food microbiology》 |2009年第8期|922-926|共5页
  • 作者

    Antonio Cobo Molinos; Hikmate Abriouel; Nabil Ben Omar; Rosario Lucas Lopez; Antonio Galvez;

  • 作者单位

    Area de Microbiologia, Departamento de Ciencias de la Salud, Facultad de Ciencias Experimentales, Universidad de Jaen, 23071-Jaen, Spain;

    Area de Microbiologia, Departamento de Ciencias de la Salud, Facultad de Ciencias Experimentales, Universidad de Jaen, 23071-Jaen, Spain;

    Area de Microbiologia, Departamento de Ciencias de la Salud, Facultad de Ciencias Experimentales, Universidad de Jaen, 23071-Jaen, Spain;

    Area de Microbiologia, Departamento de Ciencias de la Salud, Facultad de Ciencias Experimentales, Universidad de Jaen, 23071-Jaen, Spain;

    Area de Microbiologia, Departamento de Ciencias de la Salud, Facultad de Ciencias Experimentales, Universidad de Jaen, 23071-Jaen, Spain;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    bacteriocin; sprouts; culture-independent methods;

    机译：细菌素豆芽;与文化无关的方法;

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