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Discrimination of Saccharomyces cerevisiae wine strains using microsatellite multiplex PCR and band pattern analysis

机译:利用微卫星多重PCR和带谱分析区分酿酒酵母酒菌株

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We propose a rapid method for Saccharomyces cerevisiae strain identification based on multiplex PCR analysis of polymorphic microsatellite loci. Simple DNA extraction without the use of phenol, followed by a rapid PCR procedure optimised for multiplex amplification of loci SC8132X, YOR267C and SCPTSY7 and band pattern analysis of the fragments generated by agarose and polyacrylamide gel electrophoresis, has allowed us to distinguish among a panel of 30 tested commercial wine strains. This method was successfully performed in an ecological study where dominance between two strains was checked at two fermentation temperatures: 15 and 20℃.rnThe method should be useful for routine and low-budget discrimination of yeast strains, both in the wine and yeast production industries.
机译:我们提出了一种基于多态性微卫星基因座的多重PCR分析的酿酒酵母菌株鉴定的快速方法。不使用苯酚即可简单地进行DNA提取,然后通过快速PCR程序进行了优化,可对SC8132X,YOR267C和SCPTSY7基因座进行多重扩增,并对琼脂糖和聚丙烯酰胺凝胶电泳产生的片段进行带谱分析,这使我们能够区分一组30种经过测试的商品葡萄酒菌株。此方法在生态研究中成功进行,该生态研究在两个发酵温度(15和20℃)下检查了两个菌株之间的优势。rn该方法对于葡萄酒和酵母生产行业中酵母菌株的常规和低成本鉴定应该是有用的。

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