• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Food microbiology >Prevalence and characterization of Enterococcus spp. isolated from Brazilian foods
【24h】

Prevalence and characterization of Enterococcus spp. isolated from Brazilian foods

机译：肠球菌的流行和特征。从巴西食品中分离

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Enterococci can be used in the food industry as starter or probiotic cultures. However, enterococci are also implicated in severe multi-resistant nosocomial infections. In this study, the prevalence of enterococci in selected Brazilian foodstuffs (raw and pasteurized milk, meat products, cheeses and vegetables) was evaluated. Phenotypic and PCR protocols were used for species identification. Tests for production of gelatinase, haemolysin, bacteriocin and bile salt hydrolysis were done with all enterococci isolates, whereas molecular determination of virulence markers (genes esp, gel, ace, as, efaA, hyl and cylA) and antibiotic resistance was checked only for Enterococcus faecium and Enterococcus faecalis isolates. The antibiotic-resistant isolates were assayed for biofilm formation and adhesion to mammalian cells. From the 120 food samples analyzed, 52.5% were positive for enterococci, meat and cheese being the most contaminated. E. faecium was the predominant species, followed by E. faecalis, E. casseliflavus and Enterococcus gallinarum. Phenotypic tests indicated that 67.7% of isolates hydrolyzed bile salts, 15.2% produced bacteriocin, 12.0% were β-hemolytic and 18.2% produced gelatinase. Antibiotic resistance (gentamicin, tetracydine and erythromycin) and genes encoding for virulence traits were more frequent in E. faecalis than in E. faecium. Three E. faecium isolates were resistant to vancomycin. Among antibiotic-resistant isolates, 72.4% of E. faecalis were able to form biofilm and 13.8% to adhere to Caco-2 cells. Antibiotic-resistant E. faecalis and E. faecium isolates were grouped by RAPD-PCR and a scattered distribution was noted, indicating that resistance was not related to a particular clone. The spread of virulence/resistance traits in isolates of the two species and different RAPD-types suggest the pathogenic potential of both species. By contrast, the recovery of bacteriocinogenic E. faecium isolates with no virulence traits suggests their potential for biotechno-logical applications. In conclusion, our results showed that enterococci from Brazilian foods present important dualist aspects for food safety.
机译：肠球菌可在食品工业中用作发酵剂或益生菌培养物。然而，肠球菌也与严重的多耐药性医院感染有关。在这项研究中，评估了某些巴西食品（原料和巴氏灭菌牛奶，肉制品，奶酪和蔬菜）中肠球菌的患病率。表型和PCR方案用于物种鉴定。所有肠球菌分离物均进行了明胶酶，溶血素，细菌素和胆汁盐水解产物的测试，而仅对肠球菌进行了毒力标记物（esp，gel，ace，efaA，hyl和cylA基因）和抗生素抗性的分子测定。粪便和粪肠球菌分离株。测定抗生素抗性分离物的生物膜形成和对哺乳动物细胞的粘附。在分析的120个食物样本中，肠球菌阳性率为52.5％，其中肉类和奶酪污染最严重。粪肠球菌是主要的种类，其次是粪肠球菌，卡塞里夫拉弗氏菌和鸡肠球菌。表型测试表明67.7％的分离物水解了胆汁盐，15.2％产生了细菌素，12.0％是β-溶血性，18.2％产生了明胶酶。与粪便相比，粪便中的抗生素耐药性（庆大霉素，四环素和红霉素）和编码致病性状的基因更为常见。三种粪肠球菌对万古霉素具有抗性。在抗生素抗性分离株中，粪肠球菌中有72.4％能够形成生物膜，而粘附在Caco-2细胞上的占13.8％。通过RAPD-PCR将抗药性大肠埃希菌和大肠埃希菌分离株分组，并发现其散布分布，表明耐药性与特定克隆无关。毒力/抗性性状在这两种物种的分离株中的传播以及不同的RAPD类型表明这两种物种的致病潜力。相比之下，没有致病性的致病性大肠埃希菌分离株的回收表明它们具有生物技术应用潜力。总之，我们的结果表明，来自巴西食品的肠球菌对食品安全具有重要的二元性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号