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首页> 外文期刊>Food microbiology >Intraspecific genetic diversity of lactic acid bacteria from malolactic fermentation of Cencibel wines as derived from combined analysis of RAPD-PCR and PFGE patterns
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Intraspecific genetic diversity of lactic acid bacteria from malolactic fermentation of Cencibel wines as derived from combined analysis of RAPD-PCR and PFGE patterns

机译：RAPD-PCR和PFGE模式组合分析得出的Cencibel葡萄酒苹果酸乳酸发酵的乳酸菌种内遗传多样性

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摘要

Three molecular techniques, randomly amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR), pulsed field gel electrophoresis (PFGE) and differential display-polymerase chain reaction (DD-PCR) have been used to assess the intraspecific diversity of the lactic microbiota responsible for spontaneous malolactic fermentation (MLF) in Cencibel wines made at a cellar in Castilla-La Mancha (Spain). The results indicate that RAPD-PCR and PFGE are of value in typing this microbiota. Better discrimination was achieved by RAPD-PCR. Reproducibility using DD-PCR was not good, which makes this method unsuitable. Combined numerical analysis of the patterns obtained from RAPD-PCR and PFGE allowed a better discrimination; this would therefore be a suitable tool to discriminate the diversity of bacterial communities like those found in MLF of wines.rnGenetic diversity data from combined numerical analysis suggest that there is considerable microbial diversity within MLF of Cencibel wines, with some genotypes coinciding in the two vinifications analysed.
机译：三种分子技术，随机扩增的多态性DNA聚合酶链反应（RAPD-PCR），脉冲场凝胶电泳（PFGE）和差异显示聚合酶链反应（DD-PCR）已用于评估负责的乳酸菌群的种内多样性用于西班牙卡斯蒂利亚-拉曼恰酒窖生产的Cencibel葡萄酒中的自发苹果酸乳酸发酵（MLF）。结果表明，RAPD-PCR和PFGE在分型该微生物群方面具有价值。通过RAPD-PCR可以更好地区分。使用DD-PCR的重现性不好，这使该方法不合适。从RAPD-PCR和PFGE获得的模式的组合数值分析可以更好地区分；因此，这将是一个合适的工具来区分细菌群落的多样性，如葡萄酒中的微生物发酵液中发现的微生物。结合数值分析得出的遗传多样性数据表明，森奇贝尔葡萄酒的微生物发酵液中存在相当大的微生物多样性，其中两种基因型在两种酿酒中同时存在分析。

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