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首页> 外文期刊>Food microbiology >Shedding of foodborne pathogens by Caenorhabditis elegans in compost-amended and unamended soil
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Shedding of foodborne pathogens by Caenorhabditis elegans in compost-amended and unamended soil

机译：秀丽隐杆线虫在堆肥改良和未改良土壤中释放食源性病原体

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A study was done to characterize the shedding of foodborne pathogenic bacteria by Caenorhabditis elegans, evaluate the persistence of worm populations cocultured with foodborne pathogens, and determine if C. elegans disperses ingested pathogens in soil as a result of shedding. Escherichia. coli O157:H7, Salmonella enterica serotype Poona, and Listeria monocytogenes, as well as E. coli OP50, a non-pathogenic strain, were studied. Synchronous populations of C. elegans were fed for 24 h on confluent lawns of nalidixic acid-adapted bacteria. C. elegans shed viable cells of ingested bacteria on tryptic soy agar supplemented with nalidixic acid (50 μg ml~(-1)) (TSAN) throughout a 5-h post-feeding period. C. elegans persisted for up to 10 days by feeding on bacteria that had been shed and grew on TSAN. Eggs harvested from C. elegans cultured on shed foodborne pathogens had the same level of viability as those collected from C. elegans grown on shed E. coli OP50. After 6-7 days, 78%, 64%, 64%, and 76% of eggs laid by C. elegans that had fed on E. coli O157:H7, S. Poona, L. monocytogenes, and E. coli OP50, respectively, were viable. Worms fed on E. coli O157;H7 were inoculated into soil and soil amended with turkey manure compost. Populations of C. elegans persisted in compost-amended soil for at least 7 days but declined in unamended soil. E. coli O157:H7 was detected at 4 and 6 days post inoculation in compost-amended and unamended soil, and in unamended soil inoculated with E. coli OP50. Populations of E. coli O157:H7 in soil amended with turkey manure compost were significantly(α = 0.05) higher than those in unamended soil. Results indicate that C. elegans can act as a vector to disperse foodborne pathogens in soil, potentially resulting in increased risk of contaminating the surface of pre-harvest fruits and vegetables.
机译：进行了一项研究，以鉴定秀丽隐杆线虫对食源性致病菌的脱落，评估与食源性病原菌共培养的蠕虫种群的持久性，并确定秀丽隐杆线虫是否由于脱落而使摄入的病原体分散在土壤中。埃希氏菌属。研究了大肠杆菌O157：H7，肠炎沙门氏菌血清型Poona和单核细胞增生李斯特菌，以及非致病性菌株大肠杆菌OP50。线虫的同步种群在适应萘啶二酸的细菌汇合的草地上饲养了24小时。秀丽隐杆线虫在喂食后5小时内，在补充了萘啶酸（50μgml〜（-1））（TSAN）的胰蛋白酶大豆琼脂上使摄入的细菌的活细胞脱落。线虫以在TSAN上脱落并生长的细菌为食，持续长达10天。从脱落的食源性病原体上培养的秀丽隐杆线虫收获的卵与从脱落的大肠杆菌OP50上生长的秀丽隐杆线虫收集的卵具有相同水平的生存力。 6-7天后，喂食大肠杆菌O157：H7，S。Poona，单核细胞增生李斯特菌和大肠杆菌OP50的秀丽隐杆线虫产下的卵分别有78％，64％，64％和76％，分别是可行的。将以O157; H7大肠杆菌为食的蠕虫接种到土壤中，并用火鸡粪便堆肥改良土壤。秀丽隐杆线虫种群在堆肥改良土壤中持续至少7天，而在未经改良土壤中下降。接种后第4天和第6天，在堆肥改良和未改良土壤中以及在用大肠杆菌OP50接种的未改良土壤中检测到大肠杆菌O157：H7。火鸡粪肥改良土壤中的大肠杆菌O157：H7种群比未经改良土壤显着高（α= 0.05）。结果表明，秀丽隐杆线虫可以作为传播食源性病原体在土壤中的载体，从而有可能增加污染收获前水果和蔬菜表面的风险。

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