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首页> 外文期刊>Food microbiology >Analyses of the putative Crp/Fnr family of transcriptional regulators of a serotype 4b strain of Listeria monocytogenes
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Analyses of the putative Crp/Fnr family of transcriptional regulators of a serotype 4b strain of Listeria monocytogenes

机译：单核细胞增生李斯特菌血清型4b菌株的转录调节子的假定Crp / Fnr家族分析

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摘要

A whole-genome sequence analysis of Listeria monocytogenes strain F2365 revealed 15 potential members of the Crp/Fnr family of transcriptional regulatory proteins. Each gene and the flanking regions were cloned, subjected to in vitro transpositional mutagenesis, and recombined into strain F2365. Mutant strains, produced for 14 of the family members, were compared to strain F2365 for differences in carbon utilization, resistance to oxidative stress, and growth under reduced oxygen conditions that would signal an Fnr- or Crp-like function for these proteins. There were no differences among strain F2365 and the 14 mutant strains in the utilization of the carbon sources readily utilized by L. monocytogenes. Although strain KO2 had a reduced growth rate compared to strain F2365 and the other mutant strains at 30° but not at 37℃, there were no differences in growth rates among strain F2365 and the mutant strains when incubated at either 30 or 37℃ under reduced oxygen conditions. However, when compared for differences in response to oxidative stress, mutants KO2 and KO5 showed reduced oxidative stress tolerance compared to the wild-type strain F2365. These results suggest that certain members of the putative Crp/Fnr family in L. monocytogenes may function in response to oxidative stress similar to the Fnr-like protein (Flp) of other Gram-positive bacteria.
机译：李斯特菌李斯特菌F2365菌株的全基因组序列分析揭示了转录调控蛋白Crp / Fnr家族的15个潜在成员。克隆每个基因和侧翼区域，进行体外转座诱变，并重组到菌株F2365中。将针对14个家庭成员生产的突变菌株与F2365菌株进行比较，以比较碳利用，抗氧化胁迫的能力以及在降低的氧气条件下的生长情况，这些信号表明这些蛋白质具有Fnr或Crp样功能。在单核细胞增生李斯特氏菌容易利用的碳源的利用方面，菌株F2365和14个突变菌株之间没有差异。尽管与F2365菌株和其他突变菌株相比，KO2菌株在30°而非37℃时生长速率有所降低，但在30℃或37°C低温条件下孵育时，F2365菌株和突变菌株之间的生长速率没有差异。氧气条件。但是，当比较对氧化应激的响应差异时，与野生型菌株F2365相比，突变体KO2和KO5的氧化应激耐受性降低。这些结果表明，单核细胞增生李斯特氏菌中推定的Crp / Fnr家族的某些成员可能在氧化应激中起作用，类似于其他革兰氏阳性细菌的Fnr样蛋白（Flp）。

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