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Serial dilution with a confirmation step

机译:有确认步骤的连续稀释

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A serial dilution test estimates the concentration of a microbe in a broth by inoculating several tubes with portions of the broth. The test may include both presumptive and confirmation steps. For the confirmation step considered here a portion of the contents of each tube indicating a change in the presumptive step is streaked on a plate. Several colonies are selected and examined to determine if the tube contained the target microbe. A statistical model accounts for the possibility of not selecting the target microbe from a tube that contains it and a similar appearing microbe. Simulations show that this model sometimes gives estimates similar to those currently used, but at other times can make large corrections. Also, a commonness measure helps check the validity of the assumptions of this statistical model.
机译:连续稀释测试通过用部分肉汤接种几个试管来估计肉汤中微生物的浓度。测试可能包括推定步骤和确认步骤。对于此处考虑的确认步骤,每块试管中的一部分内容物指示推定步骤的变化在板上划线。选择几个菌落并检查以确定管中是否含有目标微生物。统计模型说明了不从包含目标微生物和相似微生物的试管中选择目标微生物的可能性。仿真表明,该模型有时会提供与当前使用的估计类似的估计,但在其他时候可以进行较大的校正。此外,通用性度量有助于检查此统计模型假设的有效性。

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