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首页> 外文期刊>Food Science and Biotechnology >Large-scale isolation of highly pure malaxinic acid from immature pear (Pyrus pyrifolia Nakai) fruit
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Large-scale isolation of highly pure malaxinic acid from immature pear (Pyrus pyrifolia Nakai) fruit

机译：从未成熟梨（Pyrus pyrifolia Nakai）果实中大规模分离出高纯度的草酸

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摘要

Malaxinic acid [4-(O-β-d-glucopyranosyl)-3-(3′-methyl-2′-butenyl)benzoic acid; MA] is one of the main compounds contained in pear fruit. The antifungal, antibacterial, and anticancer effects of MA have been reported. However, additional evaluations on the biological activity of MA have not been fully performed due to the lack of an MA standard. Therefore, in the present study, we established a large-scale isolation method to obtain highly pure MA from immature pear (Pyrus pyrifolia Nakai) fruit. The EtOAc-soluble acidic fraction (134.75 g) of pear fruit (18 kg fresh wt.) 60% EtOH extract (1,081.9 g) was purified by Amberlite XAD-2 (H2O/EtOH, stepwise), Diaion HP-20 (H2O/MeOH, stepwise), and Sephadex LH-20 (80% MeOH) column chromatographies. Then, the fractions containing MA were finally purified by high performance liquid chromatography using an Amide RP-C16 column on semi-preparative scale. The isolated MA (793 mg) was >99% pure, and total recovery was estimated to be 61.7%.
机译：马来酸[4-（O-β-d-吡喃葡萄糖基）-3-（3'-甲基-2'-丁烯基）苯甲酸； MA]是梨果实中包含的主要化合物之一。已经报道了MA的抗真菌，抗菌和抗癌作用。然而，由于缺乏MA标准，尚未对MA的生物活性进行额外的评估。因此，在本研究中，我们建立了一种大规模分离方法，以从未成熟梨（Pyrus pyrifolia Nakai）果实中获得高纯度的MA。通过Amberlite XAD-2（H2O / EtOH，逐步），Diaion HP-20（H2O / H2O /）纯化梨果实（18 kg新鲜重量）60％EtOH提取物（1,081.9 g）的EtOAc可溶性酸性部分（134.75 g）。 MeOH（逐步洗脱）和Sephadex LH-20（80％MeOH）色谱柱。然后，最后使用半制备规模的Amide RP-C16柱通过高效液相色谱法纯化含有MA的馏分。分离出的MA（793 mg）纯度> 99％，估计总回收率为61.7％。

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  • 来源
    《Food Science and Biotechnology》 |2013年第6期|1539-1545|共7页
  • 作者

    Yu Geon Lee; Jeong-Yong Cho; Jun Park; Sang-Hyun Lee; Wol-Soo Kim; Keun-Hyung Park; Jae-Hak Moon;

  • 作者单位

    Department of Food Science and Technology and Functional Food Research Center Chonnam National University">(1);

    Department of Food Science and Technology and Functional Food Research Center Chonnam National University">(1);

    Department of Food Science and Technology and Functional Food Research Center Chonnam National University">(1);

    Korea Pear Research Organization Chonnam National University">(2);

    Korea Pear Research Organization Chonnam National University">(2);

    Department of Horticulture Chonnam National University">(3);

    Department of Food Science and Technology and Functional Food Research Center Chonnam National University">(1);

    Department of Food Science and Technology and Functional Food Research Center Chonnam National University">(1);

    Korea Pear Research Organization Chonnam National University">(2);

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  • 正文语种 eng
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  • 关键词

    pear; Pyrus pyrifolia; immature pear fruit; malaxinic acid; high purity;

    机译：梨;梨梨;梨果实不成熟;马来酸高纯度;

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