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Optimization of phenolic compound recovery and antioxidant activity of light and dark dried fig (Ficus carica L.) varieties

机译:无花果和深色无花果品种中酚类化合物的回收率和抗氧化活性的优化

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摘要

The optimum conditions for extraction of total phenolic contents (TPC) and for enhancing the antioxidant activity from light and dark dried figs were determined. The effects of the solvent nature (acetone, ethanol, methanol, or water), solvent concentration (20–80%), acetic acid concentration (0–2%), extraction temperature (25–70°C), extraction time (0.5–4 h), sample to solvent ratio (1/25–1/100), and number of extractions (1, 2, and 3) were determined. The TPC was used to identify antioxidant compounds. 2,2-diphenyl-1-picrylhydrazyl (DPPH) was used to evaluate antioxidant activity. All extraction parameters had significant effects (p<0.05) on the TPC and the antioxidant activities. The best conditions were obtained with double extraction using 60% acetone without acidification, at 40°C for 120 min, and with a 1/75 solid to solvent ratio. These conditions resulted in TPC concentrations of 469.46 (light variety) and 399.79 mg of gallic acid (GAE)/100 g (dark variety), and antioxidant activities of 96.47 and 102.28 mg of GAE/100 g, respectively.
机译:确定了从干无花果和深色无花果中提取总酚含量(TPC)和增强抗氧化活性的最佳条件。溶剂性质(丙酮,乙醇,甲醇或水),溶剂浓度(20–80%),乙酸浓度(0–2%),萃取温度(25–70°C),萃取时间(0.5)的影响–4小时),样品与溶剂的比例(1 / 25-1 / 100)和萃取次数(1、2和3)被确定。 TPC用于鉴定抗氧化剂化合物。 2,2-二苯基-1-吡咯肼基(DPPH)用于评估抗氧化活性。所有提取参数均对TPC和抗氧化活性具有显着影响(p <0.05)。最佳条件是在不进行酸化的情况下,使用60%丙酮进行两次萃取,在40°C下120分钟,固/溶剂比为1/75。这些条件导致TPC浓度为469.46(浅色)和399.79 mg没食子酸(GAE)/ 100 g(深色),抗氧化活性分别为96.47和102.28 mg GAE / 100 g。

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