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首页> 外文期刊>Food Science and Biotechnology >β-Glycosidase-assisted bioconversion of ginsenosides in purified crude saponin and extracts from red ginseng (Panax ginseng C. A. Meyer)
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β-Glycosidase-assisted bioconversion of ginsenosides in purified crude saponin and extracts from red ginseng (Panax ginseng C. A. Meyer)

机译：β-糖苷酶辅助的人参皂苷在纯化的粗皂苷和红参提取物中的生物转化（人参C. A. Meyer）

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摘要

Major ginsenosides in ginseng (Panax ginseng) and its products are highly glycosylated, hence poorly absorbed in the gastrointestinal tract. β-Glycosidase-assisted deglycosylation of pure ginsenosides was peformed to study bioconversion mechanisms. Ginsenoside standard compounds, crude saponin, and red ginseng extracts were incubated with β-glycosidase (0.05% w/v, 55°C). β-Glycosidase has a broad specificity for β-glycosidic bonds, hydrolyzing the β-(1→6), α-(1→6), and α-(1→2) glycosidic linkages. The final metabolite of protopanaxadiol ginsenosides was Rg3 while the metabolite of protopanaxatriol ginsenosides was Rh1. β-Glycosidase treatment of red ginseng extracts resulted in a decrease in the amounts of Rb1, Rc, Re, and Rg2 after 24 h, whereas levels of the less glycosylated Rd, Rb1, Rg, Rg3, Rg1, and Rh1 forms increased. When crude saponin was incubated with β-glycosidase for 24 h, levels of Rb1, Rc, Re, and Rg1 decreased while levels of Rd, Rg3, and Rh1 increased as deglycosylated ginsenosides.
机译：人参（人参）及其产品中的主要人参皂苷高度糖基化，因此在胃肠道中吸收较差。利用β-糖苷酶辅助人参皂苷的去糖基化来研究生物转化机制。将人参皂苷标准化合物，粗制皂苷和红参提取物与β-糖苷酶（0.05％w / v，55°C）孵育。 β-糖苷酶对β-糖苷键具有广泛的特异性，可水解β-（1→6），α-（1→6）和α-（1→2）糖苷键。原泊松二醇人参皂苷的最终代谢产物为Rg3，而原泊松三醇人参皂苷的最终代谢产物为Rh1。 β-糖苷酶处理的红参提取物在24小时后导致Rb1，Rc，Re和Rg2的量减少，而较少糖基化的Rd，Rb1，Rg，Rg3，Rg1和Rh1形式的水平增加。当将粗皂苷与β-糖苷酶一起孵育24小时时，Rb1，Rc，Re和Rg1的水平会降低，而Rd，Rg3和Rh1的水平会随着去糖基化人参皂苷的增加而增加。

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