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首页> 外文期刊>Food Science and Biotechnology >Antioxidant activity of Fragilariopsis pseudonana and protective effect against hydrogen peroxide-induced inhibition of gap junctional intercellular communication
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Antioxidant activity of Fragilariopsis pseudonana and protective effect against hydrogen peroxide-induced inhibition of gap junctional intercellular communication

机译：拟南芥的抗氧化活性和对过氧化氢诱导的间隙连接细胞间通讯抑制的保护作用

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摘要

This study was aimed to evaluate antioxidant activities of a methanol extract from the polar microalgae Fragilariopsis pseudonana (FP) and to investigate the protective effect for gap junctional intercellular communication (GJIC) in WB-F344 normal rat liver epithelial cells. The results indicated that the methanol extract of FP (FPM) scavenged ABTS free radicals and inhibited copper-induced low density lipoprotein oxidation in a dose-dependent manner. Exposing HepG2 cells to FeSO4 increased intracellular reactive oxygen species formation and lipid peroxides, which was significantly reduced by treatment with FPM. Furthermore, FPM increased the activities of superoxide dismutase and glutathione peroxidase in cells. FPM recovered the hydrogen peroxide (H2O2)-induced inhibition of GJIC and attenuated the phosphorylation of connexin 43 (Cx43) and extracellular signal-regulated kinase1/2 (ERK1/2). Taken together, these results demonstrated that FPM has antioxidant activity and protects against H2O2-induced inhibition of GJIC by blocking phosphorylation of Cx43 via activation of ERK1/2.
机译：这项研究旨在评估极性微藻假鳞茎（FP）的甲醇提取物的抗氧化活性，并研究对WB-F344正常大鼠肝上皮细胞中间隙连接细胞间通讯（GJIC）的保护作用。结果表明FP（FPM）的甲醇提取物清除了ABTS自由基，并以剂量​​依赖的方式抑制了铜诱导的低密度脂蛋白的氧化。将HepG2细胞暴露于FeSO4 会增加细胞内活性氧的形成和脂质过氧化物，而FPM处理可显着减少HepG2细胞的形成。此外，FPM增加了细胞中超氧化物歧化酶和谷胱甘肽过氧化物酶的活性。 FPM回收了过氧化氢（H2 O2 ）对GJIC的抑制作用，并减弱了连接蛋白43（Cx43）和细胞外信号调节激酶1/2（ERK1 / 2）的磷酸化。综上所述，这些结果表明FPM具有抗氧化活性，并通过激活ERK1 / 2来阻断Cx43的磷酸化，从而防止H2O2 诱导的GJIC抑制。

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