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Antioxidation activity of oil extracts prepared from various seeds

机译：从各种种子制备的油提取物的抗氧化活性

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摘要

The anti-oxidative activity of ethanol and methanol extracts from various seed oils (perilla seed oil, camellia seed oil, pine seed oil, flaxseed oil, and olive oil) was investigated using DPPH free radical scavenging activity (DPPH-RSA), hydroxyl radical scavenging activity (Hydroxyl-RSA), fluorescent development, browning level development, microsomal lipid peroxidation (MLP), and t-BOOH-induced cytotoxicity assays. Both browning and fluorescence level were observed higher value in methanol extract of camellia seed oil (CSO-ME). This meant that CSO-ME had more available antioxidant matters. Radical scavenging activity of CSO-ME was the highest among all seed oil extracts, and was 61.1% in the DPPH-RSA and 64.5% in the Hydroxyl-RSA test. Based on in vitro tests, all extract treatments significantly reduced both thiobarbituric acid-reactive substrate formation and lactate dehydrogenase release, indicating high antioxidant activity. These results suggest that ethanol and methanol extracts from seed oils can be a potentially good dietary lipid supplement source against oxidative stress.
机译：使用DPPH自由基清除活性（DPPH-RSA），羟基自由基研究了各种种子油（紫苏籽油，山茶籽油，松树籽油，亚麻籽油和橄榄油）中乙醇和甲醇提取物的抗氧化活性。清除活性（羟基-RSA），荧光显影，褐变水平显影，微粒体脂质过氧化（MLP）和t-BOOH诱导的细胞毒性测定。在山茶籽油（CSO-ME）的甲醇提取物中，褐变和荧光水平均较高。这意味着CSO-ME具有更多可用的抗氧化剂物质。 CSO-ME的自由基清除活性在所有种子油提取物中最高，在DPPH-RSA中为61.1％，在Hydroxyl-RSA测试中为64.5％。根据体外试验，所有提取物处理均显着减少了硫代巴比妥酸反应性底物的形成和乳酸脱氢酶的释放，表明其高抗氧化活性。这些结果表明，从种子油中提取乙醇和甲醇可以成为抵抗氧化应激的潜在良好膳食脂质补充剂。

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