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首页> 外文期刊>Food science and technology research >Ability of Heated Scallop-Shell Powder to Disinfect Staphylococcus aureus Biofilm
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Ability of Heated Scallop-Shell Powder to Disinfect Staphylococcus aureus Biofilm

机译：扇贝加热粉对金黄色葡萄球菌生物膜的消毒能力

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The ability of heated scallop-shell powder (HSSP) to disinfect Staphylococcus aureus biofilm was investigated. A biofilm of S. aureus NBRC 13276 was produced on a glass plate. Scallop-shell powder was exposed to heat treatment at 1000℃ in air for 1 h, followed by immersion of the biofilm plate into the HSSP slurry. After treatment, the disinfection ability of the HSSP toward the biofilm was measured non-destructively and quantitatively using a conductometric assay. Disinfection efficacy against S. aureus biofilm increased with HSSP concentration and treatment time. HSSP treatment (10 mg/mL, pH 12.5) of 60 min completely eliminated the biofilm bioactivity (which was approximately 10~7 CFU/cm~2). In contrast, treatment with NaOH solution (pH 12.5) at a pH identical to the HSSP slurry and treatment with sodium hypochlorite (200 μg/mL) caused an approximately four- to five-order reduction in activity. Fluorescence microscopy confirmed that no viable cells remained on the plate after HSSP treatment. Although alkaline and sodium hypochlorite treatments had a stripping effect on the biofilm, many viable cells remained on the plate. HSSP exerted a disinfection effect against S. aureus biofilm that was greater than that provided by alkaline or sodium hypochlorite treatment. This disinfection effect can be effective for controlling bacteria responsible for food poisoning. The use of HSSP in food processing is expected to not only be a source of minerals but also prolong the shelf-life of foodstuffs and reduce the pollution resulting from discarded shells.
机译：研究了加热的扇贝贝壳粉（HSSP）对金黄色葡萄球菌生物膜的消毒能力。在玻璃板上产生金黄色葡萄球菌NBRC 13276的生物膜。扇贝壳粉在空气中于1000℃下进行热处理1 h，然后将生物膜平板浸入HSSP浆料中。处理后，使用电导测定法无损和定量地测量了HSSP对生物膜的消毒能力。随HSSP浓度和处理时间的增加，对金黄色葡萄球菌生物膜的消毒功效也随之增加。 60分钟的HSSP处理（10 mg / mL，pH 12.5）完全消除了生物膜的生物活性（约10〜7 CFU / cm〜2）。相反，用与HSSP浆液相同的pH值的NaOH溶液（pH 12.5）处理和用次氯酸钠（200μg/ mL）处理导致活性降低约四至五级。荧光显微镜证实在HSSP处理后板上没有活细胞残留。尽管碱性和次氯酸钠处理对生物膜具有剥离作用，但板上仍有许多活细胞。 HSSP对金黄色葡萄球菌生物膜的消毒作用大于碱性或次氯酸钠处理所提供的消毒作用。这种消毒作用可以有效地控制引起食物中毒的细菌。预计在食品加工中使用HSSP不仅是矿物质的来源，而且可以延长食品的保质期，并减少因丢弃的贝壳而造成的污染。

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