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首页> 外文期刊>Food science and technology research >In Vitro Antioxidant and Antitumor Activities of Polysaccharides Extracted from the Mycelia of Liquid-Cultured Flammulina velutipes
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In Vitro Antioxidant and Antitumor Activities of Polysaccharides Extracted from the Mycelia of Liquid-Cultured Flammulina velutipes

机译：液体培养金针菇菌丝体多糖的体外抗氧化和抗肿瘤活性

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摘要

Orthogonal experiment was used to optimize the extraction conditions of Flammulina velutipes mycelia polysaccharides (FvP). Four independent variables (ratio of water to raw material, initial pH value, extracting temperature, and extracting time) were taken into consideration. A yield of FvP of 2.19% was obtained under an optimized condition (ratio of water to material of 50:1, initial pH value of 6.0, extracting temperature of 85℃, and extracting time of 6 h). Subsequently, antioxidative properties of FvP-2 (crude polysaccharides) and FvP-3 (deproteinized polysaccharides) prepared from E velutipes mycelia were evaluated by monitoring the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical and hydroxyl radical scavenging activity, together with antitumor activity against the human hepatocellular carcinoma BEL-7402 cells. DPPH scavenging rate of 65.85% and hydroxyl radical scavenging rate of 71.24% were obtained at 200 μg/mL of FvP-3. The inhibition rate of BEL-7402 cells was up to 45% at 640 μg/mL of FvP-2. These results suggested that FvP possesses potent antioxidant and antitumor properties. The polysaccharide may be useful as a functional food additive and an antioxidant and antitumor agent.
机译：正交试验优化了金针菇菌丝体多糖（FvP）的提取条件。考虑了四个独立变量（水与原料的比例，初始pH值，萃取温度和萃取时间）。在最佳条件下（水与物料之比为50：1，初始pH值为6.0，萃取温度为85℃，萃取时间为6 h），FvP的产率为2.19％。随后，通过监测2,2-二苯基-1-吡啶并肼基（DPPH）自由基和羟自由基清除活性，评估了从velutipes菌丝体制备的FvP-2（粗多糖）和FvP-3（脱蛋白多糖）的抗氧化性能，以及针对人类肝细胞癌BEL-7402细胞的抗肿瘤活性。在200μg/ mL的FvP-3条件下，DPPH清除率为65.85％，羟自由基清除率为71.24％。在640μg/ mL FvP-2下，BEL-7402细胞的抑制率高达45％。这些结果表明FvP具有有效的抗氧化和抗肿瘤特性。多糖可用作功能性食品添加剂以及抗氧化剂和抗肿瘤剂。

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  • 来源
    《Food science and technology research》 |2013年第4期|661-667|共7页
  • 作者

    Chao Zhao; Kun Zhao; Xiaoyan Liu; Yi-fan Huang; Bin Liu;

  • 作者单位

    College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China ,National Engineering Research Center ofJuncao, Fuzhou, Fujian 350002, China;

    College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;

    College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;

    College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China ,National Engineering Research Center ofJuncao, Fuzhou, Fujian 350002, China ,College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;

    College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China ,National Engineering Research Center ofJuncao, Fuzhou, Fujian 350002, China;

  • 收录信息 美国《科学引文索引》(SCI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Flammulina velutipes; mycelial fermentation; polysaccharides; antioxidant; antitumor;

    机译：金针菇;菌丝发酵多糖;抗氧化剂抗肿瘤;

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