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Chemical Nature of a New Antihypertensive Peptide Derived from Jellyfish

机译:一种来自海New的新型降压肽的化学性质

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摘要

A high-concentration (20%) antihypertensive peptide solution with angiotensin I-converting enzyme (ACE) inhibitory activity was prepared from the powder of the jellyfish Stomolophus nomurai, a plague of which had recently hit Japan's fishing industry, using the drum dryer method. Three active fractions, Fr. 3-1, Fr. 3-3, and Fr. 3-6, were isolated from the peptide mixture by step-wise elution by Sep-Pak Vac C_(18) and further fractionated by high-performance liquid chromatography twice. The sequences of Fr. 3-1, Fr. 3-3, and Fr. 3-6 were determined as IGDEPLANYL, FGGIDDINQIGQSD, and YYAPFE, respectively. The most active peptide was identified with the synthetic peptide YYAPFE by sequence analysis, MALDI-TOF MS, and NMR spectra. Further, YYAPFQ, designed and synthesized on the basis of YYAPFE, showed the highest ACE inhibitory activity in vitro among YYAPFE, YYAPF, YYAP, YYA, and YY; however, its effect on systolic blood pressure showed a similar tendency to that of YYAPFE in spontaneously hypertensive rats.
机译:使用鼓式烘干机方法,从最近在日本捕鱼业遭受灾难的海el Stomolophus nomurai的粉末中制备具有高浓度(20%)的具有血管紧张素I转换酶(ACE)抑制活性的降压肽溶液。三个活性成分Fr。 3-1,神父3-3和Fr。通过Sep-Pak Vac C_(18)逐步洗脱从肽混合物中分离出3-6,并通过高效液相色谱进一步分离两次。 Fr的序列。 3-1,神父3-3和Fr。将3-6确定为IGDEPLANYL,FGGIDDINQIGQSD和YYAPFE。通过序列分析,MALDI-TOF MS和NMR光谱,用合成肽YYAPFE鉴定出活性最高的肽。此外,在YYAPFE的基础上设计合成的YYAPFQ在YYAPFE,YYAPF,YYAP,YYA和YY中表现出最高的ACE抑制活性。然而,它对自发性高血压大鼠收缩压的影响与YYAPFE相似。

著录项

  • 来源
    《Food science and technology research》 |2010年第4期|P.333-340|共8页
  • 作者单位

    Graduate School of Health Sciences, Aomori University of Health and Welfare, 58-1 Mase, Hamadate, Aomori, Aomori 030-8505, Japan;

    rnGraduate School of Health Sciences, Aomori University of Health and Welfare, 58-1 Mase, Hamadate, Aomori, Aomori 030-8505, Japan;

    rnShimokita Food Research Institute, Aomori Prefectural Industrial Technology Research Center, 154 Uwano, Ohata, Mutsu, Aomori 039-4401, Japan;

    rnFaculty of Pharmacy, Meijo University, 150 Yagotoyama, Tempaku, Nagoya, Aichi 468-8503, Japan;

    rnAomori Prefectural Industrial Technology Research Center, 82-9 Tanaka, Kuroishi, Aomori 036-0522, Japan;

    rnGraduate School of Health Sciences, Aomori University of Health and Welfare, 58-1 Mase, Hamadate, Aomori, Aomori 030-8505, Japan;

  • 收录信息 美国《科学引文索引》(SCI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    jellyfish; peptide; antihypertensive; drum dryer; structure; activity;

    机译:海蜇;肽降压滚筒烘干机结构体;活动;

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