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Monitoring of efficacy of antimicrobial products during 60 days storage simulation of diesel (B0), biodiesel (B100) and blends (B7 and B10)

机译：在模拟柴油（B0），生物柴油（B100）和混合物（B7和B10）的60天存储期间监控抗菌产品的功效

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摘要

Microorganisms can cause many operational problems, particularly, during storage and handling of fuel systems. The susceptibility of diesel systems to microbial contamination has been studied for many years but the introduction of biodiesel (Brazil- B5) has raised the incidence of problems in tanks around the world. Among the mitigation alternatives, biocides have been identified as a good one to curb microbial growth. The aim of this research was the effectiveness assessment of two biocides a MBO antimicrobial agent (as multifunctional package) and MIT/CMIT antimicrobial agent in biodiesel (B100 - 60% soya and 40% tallow), conventional diesel (BO - low sulfur 50 ppm) and blends B7 and B10. The efficacy of two biocides was determined by evaluating the changes in a set of parameters during 60-days exposure to an uncharacterized microbial inoculum. Microcosms contained a fuel phase (BO, B100, B7 or B10) and two types of aqueous phase: natural bottom-water formed in B5 storage tank or a synthetic water with three levels of contamination: low (10~3 CFU IT~(-1)), medium (10~5 CFU mL~(-1)) and high (10~8 CFU mL~(-1)). Fuel phase as received and without biocide with sterile aqueous phase was used as a control and the sampling times were at 0, 7,14, 21, 42 and 60 days. The fuel phase was rated by Haze scale (ASTMD 4176) and infrared analysis. Water phase parameters included: microbial viability (time-kill), presence of emulsion/biofilm and dry weight of biomass formed at fuel/water interface. The results suggest that antimicrobial MBO product as a multifunctional package can only control the microbial population when the microbial contamination level is low. The antimicrobial MIT/CMIT product was effective in all conditions (as received, media and high microbial contamination).
机译：微生物会引起许多操作问题，尤其是在燃料系统的存储和处理过程中。柴油系统对微生物污染的敏感性已经研究了很多年，但是生物柴油（巴西B5）的引入增加了世界范围内储罐中问题的发生率。在缓解措施中，已确认杀生物剂是抑制微生物生长的良好方法。这项研究的目的是评估两种生物杀灭剂（生物柴油中的MBO抗菌剂（作为多功能包装）和MIT / CMIT抗菌剂（B100-大豆含量为60％和牛脂含量为40％）），常规柴油（BO-低硫含量为50 ppm ）并混合B7和B10。通过评估暴露于未经鉴定的微生物接种物60天期间一组参数的变化来确定两种杀菌剂的功效。微观世界包含燃料相（BO，B100，B7或B10）和两种水相：B5储罐中形成的天然底水或三种污染程度的合成水：低（10〜3 CFU IT〜（- 1）），中等（10〜5 CFU mL〜（-1））和高（10〜8 CFU mL〜（-1））。接收到的燃料相和没有杀生物剂的无菌水相用作对照，采样时间为0、7、14、21、42和60天。通过雾度标度（ASTMD 4176）和红外分析对燃料相进行评级。水相参数包括：微生物生存力（杀灭时间），乳剂/生物膜的存在以及在燃料/水界面形成的生物质的干重。结果表明，当微生物污染水平较低时，作为多功能包装的抗菌MBO产品只能控制微生物种群。抗菌MIT / CMIT产品在所有条件下均有效（原样，培养基和高微生物污染）。

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  • 来源
    《Fuel》 |2013年第10期|153-162|共10页
  • 作者

    A. Zimmer; J. Cazarolli; R.M. Teixeira; S.LC. Viscardi; E.S.H. Cavalcanti; A.E. Gerbase; M.F. Ferrao; C.M.S. Piatnicki; F.M. Bento;

  • 作者单位

    Unlversidade Federal do Rio Grande do Sul Microbiology, Immunology and Parasitology Department, RS, Brazil;

    Unlversidade Federal do Rio Grande do Sul Microbiology, Immunology and Parasitology Department, RS, Brazil;

    Ipiranga Produtos de Petroleo, RJ, Brazil;

    Ipiranga Produtos de Petroleo, RJ, Brazil;

    Institute National de Tecnologia - INT, Corrosion and Protection Laboratory/Lacor, RJ, Brazil;

    Universidade Federal do Rio Grande do Sul, Chemistry Department, RS, Brazil;

    Universidade Federal do Rio Grande do Sul, Chemistry Department, RS, Brazil;

    Universidade Federal do Rio Grande do Sul, Chemistry Department, RS, Brazil;

    Unlversidade Federal do Rio Grande do Sul Microbiology, Immunology and Parasitology Department, RS, Brazil;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Biodiesel; Blends; Biological sludge; Antimicrobial product; Additive;

    机译：生物柴油混合;生物污泥;抗菌产品;添加剂;

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