• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Genes & Genomics >Microarray analysis of gene expression in peripheral blood leucocytes from rock bream (Oplegnathus fasciatus) after stimulation by LPS, ConA/PMA, and poly I:C
【24h】

Microarray analysis of gene expression in peripheral blood leucocytes from rock bream (Oplegnathus fasciatus) after stimulation by LPS, ConA/PMA, and poly I:C

机译：LPS，ConA / PMA和poly I：C刺激后，岩鲷（Oplegnathus fasciatus）外周血白细胞基因表达的微阵列分析

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The responses of peripheral blood leucocytes (PBLs) from the rock bream Oplegnathus fasciatus stimulated with lipopolysaccharides (LPS), concanavalin A/phorbol myristate acetate (Con A/PMA), and/or polyriboinosinic polyribocytidylic acid (poly I:C) were investigated using a cDNA microarray consisting of 2,400 clones. A total of 336 unique genes were more than twofold up-regulated: 169 in LPS-stimulated PBLs, 209 in Con A/PMA-stimulated PBLs, and 146 in poly I:C-stimulated PBLs. Moreover, other genes were similarly up-regulated with combinations of stimulants: 85 genes in PBLs stimulated with both LPS and Con A/PMA, 79 in PBLs stimulated with both LPS and poly I:C, and 66 in PBLs stimulated with both Con A/PMA and poly I:C. Furthermore, 42 other genes were more than twofold up-regulated in PBLs stimulated with all tested mitogens. Other genes were not significantly induced. Overall, these results suggest that certain genes in rock bream play important roles in physiological and pathological processes. Furthermore, the microarray analysis showed promise as a tool for investigating immune mechanisms in teleost fish.
机译：研究了脂多糖（LPS），伴刀豆球蛋白A /佛波醇肉豆蔻酸酯乙酸盐（Con A / PMA）和/或多核糖核酸聚核糖酸（poly I：C）刺激的岩鲷Oplegnathus fasciatus外周血白细胞（PBL）的反应。由2,400个克隆组成的cDNA微阵列。共有336个独特基因被上调了两倍以上：LPS刺激的PBL中为169个，Con A / PMA刺激的PBL中为209个，poly I：C刺激的PBL中为146个。此外，其他基因也同样受到刺激物组合的上调：LPS和Con A / PMA共同刺激的PBL中有85个基因，LPS和poly I：C共同刺激的PBL中有79个基因，Con A两者刺激的PBL中有66个基因。 / PMA和poly I：C。此外，在所有测试有丝分裂原刺激的PBL中，还有42个其他基因上调了两倍以上。其他基因没有被明显诱导。总体而言，这些结果表明，鲷鱼中的某些基因在生理和病理过程中起着重要作用。此外，微阵列分析显示了有望作为研究硬骨鱼免疫机制的工具。

著录项

  • 来源
    《Genes & Genomics》 |2013年第3期|343-353|共11页
  • 作者

    Mun-Gyeong Kwon; Ju-Won Kim; Myoung-Ae Park; Jee-Youn Hwang; Hye-Sung Choi; Mu-Chan Kim; Dae-Won Park; J.-M. Jung; Chan-Il Park;

  • 作者单位

    Pathology Division National Fisheries Research and Development Institute">(1);

    Institute of Marine Industry College of Marine Science Gyeongsang National University">(2);

    Pathology Division National Fisheries Research and Development Institute">(1);

    Pathology Division National Fisheries Research and Development Institute">(1);

    Pathology Division National Fisheries Research and Development Institute">(1);

    Institute of Marine Industry College of Marine Science Gyeongsang National University">(2);

    Fisheries Resources Research Institute">(3);

    Institute of Marine Industry College of Marine Science Gyeongsang National University">(2);

    Institute of Marine Industry College of Marine Science Gyeongsang National University">(2);

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Rock bream; PBLs; LPS; Con A/PMA; Poly I:C; Microarray analysis;

    机译：鲷鱼PBL;LPS;Con A / PMA;保利I：C;芯片分析;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号