A tandem-repeat galectin from blood clam Tegillarca granosa and its induced mRNA expression response against bacterial challenge

Yongbo Bao; Haoping Shen; Huashan Zhou; Yinghui Dong; Zhihua Lin

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A tandem-repeat galectin from blood clam Tegillarca granosa and its induced mRNA expression response against bacterial challenge

机译：血蛤Tegillarca granosa的串联重复半乳糖凝集素及其诱导的针对细菌攻击的mRNA表达反应

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Galectins are a family of β-galactoside-binding lectins which play crucial roles in innate immunity of vertebrates and invertebrates. In the present study, we have cloned and characterized the first galectin to be identified in Tegillarca granosa (designated Tg-GAL). The full-length cDNA of Tg-GAL was of 2,394 bp nucleotides, encoding a polypeptide of 354 amino acids. The amino acid sequence of T. granosa galectin (Tg-GAL) showed striking sequence similarity to invertebrate and vertebrate galectins in carbohydrate recognition domains (CRD) and contained amino acids that are crucial for binding β-galactoside sugars. Structurally, the Tg-GAL was a tandem repeat galectin containing two CRD connected by a unique peptide link. Quantitative real-time PCR was employed to investigate the tissue distribution of Tg-GAL mRNA and temporal expression in haemocytes of clams challenged with Vibrio parahaemolyticus, lipopolysaccharide (LPS) and peptidogylcan (PGN). The Tg-GAL mRAN expression was concentrated in hepatopancreas and mantle. The up-regulation of Tg-GAL after bacteria V. parahaemolyticus, LPS and PGN challenge showed that Tg-GAL might play a pivotal role in anti-bacterial immunity. Further study should investigate the effects of Tg-GAL absence by siRNA knockout.

机译：半乳凝素是β-半乳糖苷结合凝集素家族，在脊椎动物和无脊椎动物的先天免疫中起着至关重要的作用。在本研究中，我们已经克隆并鉴定了在Tegillarca granosa（指定为Tg-GAL）中鉴定的第一个半乳糖凝集素。 Tg-GAL的全长cDNA为2,394 bp核苷酸，编码354个氨基酸的多肽。 T. granosa galectin（Tg-GAL）的氨基酸序列与碳水化合物识别域（CRD）中的无脊椎动物和脊椎动物半乳糖凝集素具有惊人的序列相似性，并且包含对于结合β-半乳糖苷糖至关重要的氨基酸。在结构上，Tg-GAL是一个串联重复半乳糖凝集素，含有两个通过独特的肽键连接的CRD。实时定量PCR用于研究Tg-GAL mRNA的组织分布和在副溶血性弧菌，脂多糖（LPS）和肽基葡聚糖（PGN）攻击的蛤的血细胞中的瞬时表达。 Tg-GAL mRAN表达集中在肝胰腺和地幔中。副溶血弧菌，LPS和PGN攻击后Tg-GAL的上调表明Tg-GAL可能在抗菌免疫中起关键作用。进一步的研究应研究siRNA敲除对Tg-GAL缺失的影响。

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来源
《Genes & Genomics》 |2013年第6期|733-740|共8页
作者
Yongbo Bao; Haoping Shen; Huashan Zhou; Yinghui Dong; Zhihua Lin;
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Zhejiang Key Lab of Aquatic Germplasm Resource Zhejiang Wanli University">(1);

Zhejiang Key Lab of Aquatic Germplasm Resource Zhejiang Wanli University">(1);

Zhejiang Key Lab of Aquatic Germplasm Resource Zhejiang Wanli University">(1);

Zhejiang Key Lab of Aquatic Germplasm Resource Zhejiang Wanli University">(1);

Zhejiang Key Lab of Aquatic Germplasm Resource Zhejiang Wanli University">(1);

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正文语种 eng
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Tegillarca granosa; Galectin; Vibrio parahaemolyticus; LPS; PGN; qRT-PCR; Immune response;

机译：Tegillarca granosa;半乳凝素;副溶血性弧菌;LPS;PGN;定量RT-PCR免疫反应;

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专利

1. A tandem-repeat galectin from blood clam Tegillarca granosa and its induced mRNA expression response against bacterial challenge [J] . Yongbo Bao, Haoping Shen, Huashan Zhou, Genes and genomics . 2013,第6期

机译：血蛤Tegillarca granosa的串联重复半乳糖凝集素及其诱导的针对细菌攻击的mRNA表达反应
2. cDNA cloning and mRNA expression of heat shock protein 70 gene in blood clam Tegillarca granosa against heavy metals challenge [J] . Jun Zhou, Chenghua Li, Ye Li, African Journal of Biotechnology . 2013,第18期

机译：血蛤Tegillarca granosa抗重金属攻击热休克蛋白70基因的cDNA克隆及mRNA表达。
3. Hemoglobin of the bloody clam Tegillarca granosa (Tg-HbI) is involved in the immune response against bacterial infection [J] . Bao Yongbo, Wang Qing, Lin Zhihua . Fish & Shellfish Immunology . 2011,第4期

机译：血红蛤Tegillarca granosa（Tg-HbI）的血红蛋白参与针对细菌感染的免疫反应
4. Histopathological alternations of blood clam Tegillarca granosa in acute copper, zinc, lead and cadmium exposures [C] . Guangxu Liu, Xueliang Chai, Yanqing Shao, ICEEP 2012 . 2012

机译：血清Tegillarca granosa在急性铜，锌，铅和镉暴露中的组织病理学交替
5. Blood sample processing for the study of aging, and characterization of caspasemRNA expression in peripheral blood mononuclear cells. [D] . Lacelle, Chantale. 2003

机译：血液样品处理，用于研究衰老以及外周血单核细胞中caspasemRNA表达的特征。
6. Identification of a regulation network in response to cadmium toxicity using blood clam Tegillarca granosa as model [O] . Yongbo Bao, Xiao Liu, Weiwei Zhang, -1

机译：使用血蛤Tegillarca granosa作为模型识别对镉毒性的调节网络
7. Erratum to: Genomic Insights into the Origin and Evolution of Molluscan Red-Bloodedness in the Blood Clam Tegillarca granosa [O] . Yongbo Bao, Qifan Zeng, Jing Wang, 2021

机译：错误：基因组洞察软体动物红细胞在血液蛤蜊Tegillarca Granosa中的起源和演变

A tandem-repeat galectin from blood clam Tegillarca granosa and its induced mRNA expression response against bacterial challenge

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