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Molecular genetic diversity analysis in emmer wheat (Triticum dicoccon Schrank) from India

机译:来自印度的Emmer小麦(Triticum dicoccon Schrank)的分子遗传多样性分析

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Emmer wheat (Triticum dicoccon Schrank) is still largely cultivated in India, and highly appreciated for the preparation of traditional dishes. Moreover, its nutritional characteristics could justify a development of its cultivation. The perspective of genetic improvement however requires a good knowledge of the genetic diversity existing within the eco-geographic group of Indian emmer wheats. A set of 48 emmer wheat accessions from India including 28 from a local collection and 20 Indian accessions obtained from CIMMYT, Mexico, was assessed for genetic variability using 47 microsatellite (SSR) markers, distributed over all the 14 chromosomes. The number of alleles per locus ranged from 2 to 9, with an average of 3.87 alleles per locus. A total of 201 alleles were detected at 52 loci with average polymorphic information content of 0.35 per locus and a mean resolving power of 1. The pair-wise similarity coefficients calculated from binary data matrix based on presence or absence of alleles varied from 0.15 to 0.98, but was greater than 0.5 for most accessions, indicating a high level of similarity. A cluster analysis based on the similarity matrix identified nine distinct accessions and three clusters. All the recently developed commercial varieties were distinctly different from the clusters. Based on the analysis, it appears that Indian emmer wheats are not very diverse. Consequently, there is a need to increase the diversity within the Indian emmer wheat eco-geographic group, by introducing diversity from other eco-geographic groups, or even from other wheat species.
机译:Emmer小麦(Triticum dicoccon Schrank)仍在印度大量种植,并因其制作传统菜肴而受到高度赞赏。此外,其营养特性可以证明其发展的合理性。然而,从遗传改良的角度出发,需要对印度Emmer小麦的生态地理群内存在的遗传多样性有充分的了解。使用分布在所有14条染色体上的47个微卫星(SSR)标记对一组来自印度的48个小麦小麦种质进行了评估,其中包括28个本地小麦种质和从墨西哥CIMMYT获得的20个印度种质的遗传变异性。每个基因座的等位基因数量范围为2至9,平均每个基因座为3.87个等位基因。在52个基因座处总共检测到201个等位基因,平均每个位点的多态信息含量为0.35,平均分辨力为1。基于等位基因存在或不存在的二进制数据矩阵计算的成对相似系数在0.15到0.98之间变化。 ,但对于大多数登录而言,该值均大于0.5,表明高度相似。基于相似度矩阵的聚类分析确定了九个不同的种质和三个聚类。所有最近开发的商业品种都与集群明显不同。根据该分析,看来印度Emmer小麦不是很多样化。因此,有必要通过引入其他生态地理群体甚至其他小麦物种的多样性来增加印度emmer小麦生态地理群体的多样性。

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