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首页> 外文期刊>Genetic Resources and Crop Evolution >Molecular differentiation in Indian Citrus L. (Rutaceae) inferred from nrDNA ITS sequence analysis
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Molecular differentiation in Indian Citrus L. (Rutaceae) inferred from nrDNA ITS sequence analysis

机译：从nrDNA ITS序列分析推断印度柑橘（芸香科）的分子分化

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摘要

Molecular differentiation in 24 accessions representing 19 taxa of Indian Citrus has been examined through sequence analysis of Internal Transcribed Spacer (ITS) region of nrDNA. Sequence length in the 24 accessions of Citrus taxa ranged from 512 to 665 bp (ITS1 & ITS2 partial and 5.8S complete sequence). The ITS sequences were very rich in G+C content ranging from 61.40 to 66.60% with an average of 64.2%. Genetic distance within Citrus group ranged from 0 to 13.4% with an average of 4.6%, showing moderate rate of nucleotide divergence. The phylogeny was inferred using the Maximum parsimony (MP) and Neighbor-Joining (NJ) methods. Both MP and NJ trees separated all the 24 accessions of Citrus into six distinct clusters. The disposition of all the accessions of Citrus in separate clusters in ITS-derived dendrograms was partly in accordance with the morpho-taxonomic affinities of the target taxa. This study supports the concept of Citrus medica (citron), C. reticulata (mandarin), and C. maxima (pummelo) as the basic species of the genus. However, ITS marker could not find any clear cut differentiation between subgenera Citrus and Papeda as proposed in Swingle’s Citrus classification system. The present study also supports the distinctiveness of C. indica (Indian wild orange), C. latipes (Khasi papeda) and C. hystrix (Melanesian papeda) as true species, besides elucidating the probable hybrid origin and relationships among the cultivated species/biotypes, such as Citrus ×aurantiifolia (sour lime) C. ×limon (lemon), C. ×taitensis (Indian rough lemon), C. limettioides (sweet lime), C. ×aurantium (including sour and sweet oranges and grapefruit), and other indigenous varieties of Indian origin: C. megaloxycarpa (sour pummelo), C. karna (karna orange), C. pseudolimon (Hill lemon), ‘Memang athur’, ‘Pummelo-lemon’ and ‘Kathairi nimbu’.
机译：通过对nrDNA的内部转录间隔区（ITS）区域进行序列分析，研究了代表19个印度柑橘类群的24个种质的分子分化。 24种柑橘类群的序列长度范围为512至665bp（ITS1和ITS2部分序列和5.8S完整序列）。 ITS序列的G + C含量非常丰富，范围为61.40％至66.60％，平均为64.2％。 Citrus组内的遗传距离范围为0至13.4％，平均为4.6％，显示出中等程度的核苷酸差异。使用最大简约法（MP）和邻居加入法（NJ）推断系统发育。 MP和NJ树将柑橘的所有24个种均分为六个不同的簇。在ITS衍生的树状图中，将所有柑橘的种质布置在单独的簇中，部分原因是根据目标分类单元的形态分类学亲和力。这项研究支持了柑橘属（Citrus medica），柑桔网（C. reticulata）（普通话）和美洲柑桔（C. maxima）（柚子）作为该属的基本物种的概念。但是，如Swingle的Citrus分类系统中所建议的那样，ITS标记无法在Citrus子属和Papeda属之间找到任何明确的区分。除阐明可能的杂种起源和栽培物种/生物型之间的关系外，本研究还支持印度C（印度野生橙），拉提比丝（Khasi papeda）和南美。（Melanesian papeda）的独特性。 ，例如柑桔×酸橙（C.×柠檬）（柠檬），C.×taitensis（印度粗柠檬），C. limettioides（甜石灰），C。×铀（包括酸橙和甜橙以及葡萄柚），以及其他印度土著品种：C。megaloxycarpa（酸柚），C。karna（卡纳橙），C。pseudolimon（山柠檬），“ Memang athur”，“ Pummelo-柠檬”和“ Kathairi nimbu”。

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