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首页> 外文期刊>Genetic Resources and Crop Evolution >Morphological and AFLP diversity in Thaumatococcus daniellii, the source of the protein sweetener thaumatin
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Morphological and AFLP diversity in Thaumatococcus daniellii, the source of the protein sweetener thaumatin

机译:蛋白质甜味剂thaumatin的来源达氏梭菌的形态和AFLP多样性

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摘要

Thaumatococcus daniellii is a perennial African wild edible plant. Fruit arils from natural populations are the source of the protein sweetener, thaumatin. There are relatively few documented examples of its cultivation, however it has great potential as an intercrop for cultivation under rubber or cocoa. Across the species range there is notable diversity in leaf and fruit morphology. In this study, T. daniellii populations from four provenances in Ghana and Cameroon (separated by the Dahomey Gap) were compared for leaf and fruit morphology in situ and in an experiment using plants grown from sampled rhizomes planted under rubber in a single location in Cameroon. When collected rhizomes from four provenances were planted at one location, the samples from the local provenance produced the largest plants and fruits, and there was less morphological variation between samples from different provenances than was recorded in situ. In situ morphological differences were notable between north–south separated provenances in each country. Fruit collected in situ was analysed for aril thaumatin content and significant variation was found between provenances (1.08–2.00%). Amplified fragment length polymorphism (AFLP) was used to assess genetic diversity among the four provenances and 54% of loci were polymorphic. Cluster analysis of AFLP data separated samples of T. daniellii according to geographical origin. Maximum genetic diversity was found between provenances separated across the Dahomey Gap.
机译:达尼微球菌是非洲多年生野生食用植物。来自自然种群的假种皮是蛋白质甜味剂thaumatin的来源。关于它的栽培的文献记载相对较少,但是作为橡胶或可可栽培的间作作物具有很大的潜力。在整个物种范围内,叶片和果实的形态具有明显的多样性。在这项研究中,比较了来自加纳和喀麦隆(由达荷美差距)分离的四个种源的丹尼氏球菌种群的原位和果实形态,并在一项实验中使用了在喀麦隆单一地点橡胶下种植的采样根茎上种植的植物。 。当从一个地方种下四个来源的根茎时,当地来源的样品产生的植物和果实最大,不同来源的样品之间的形态变化比原地记录的要少。在每个国家中,南北分离种源之间的原位形态差异十分明显。对原位收集的水果进行了芦荟甜蛋白含量的分析,发现不同来源之间的差异很大(1.08–2.00%)。扩增片段长度多态性(AFLP)用于评估四个来源中的遗传多样性,且54%的基因座是多态性。 AFLP数据的聚类分析根据地理来源分离了丹氏梭菌的样本。在跨越达荷美差距的种源之间发现了最大的遗传多样性。

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