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Chemotypical variation in Vanilla planifolia Jack. (Orchidaceae) from the Puebla-Veracruz Totonacapan region

机译:香草平叶杰克的化学型变异。 (兰科)来自普埃布拉-韦拉克鲁斯州Totonacapan地区

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One of the threats in the diversity loss of the primary gene pool of Vanilla planifolia is the lack of information on existing level of polymorphism in cultivated germplasm, and the different expressions of this polymorphism. For this reason, it is proposed to study the chemical polymorphism of the four phytochemicals that define the vanilla aroma quality in fruits (vanillin, vanillic acid, p-hydroxybenzaldehyde, p-hydroxybenzoic acid) by HPLC analysis (High Performance Liquid Chromatography) of 25 collections of unknown genotype, grown in the region Totonacapan Puebla-Veracruz, Mexico. The results identified a selection process, domestication in fruit aroma of vanilla, during which increased the participation of vanillin and reduced the presence of three minor compounds (vanillic acid, p-hydroxybenzaldehyde and p-hydroxybenzoic acid) in the global aroma. We distinguished a total of six chemotypes of V. planifolia in the Totonacapan region, some chemotypes with wild aromatic characteristics (low participation of vanillin) related to the material less cultivated in the region and domesticated chemotypes with high participation of vanillin, for the most cultivated material. The results show that the diversification of the chemotypes of V. planifolia is not related to environmental variation. The data indicate that in the possible center of origin of vanilla, there is phytochemical polymorphism, which indirectly suggests the existence of genetic polymorphism, essential for the design of a breeding program for optimizing the use and conservation of diversity of the primary gene pool of Vanilla planifolia.
机译:平原香草初级基因库多样性丧失的威胁之一是缺乏关于栽培种质中现有多态性水平的信息以及该多态性的不同表达。因此,建议通过25的HPLC分析(高效液相色谱)研究定义水果中香草香气质量的四种植物化学成分的化学多态性(香兰素,香草酸,对羟基苯甲醛,对羟基苯甲酸)。墨西哥Totonacapan Puebla-Veracruz地区生长的未知基因型的病原菌。结果确定了一种选择过程,即在香草的水果香气中驯化,在此过程中,香兰素的参与度增加,并且减少了全球香气中三种次要化合物(香草酸,对羟基苯甲醛和对羟基苯甲酸)的存在。我们区分了Totonacapan地区的六个V. Planifolia化学型,其中一些具有野生芳香特性的化学型(香草醛的参与度低)与该地区较少种植的材料有关,而驯化的化学成分的香草醛的参与度最高,材料。结果表明,V。Planifolia的化学型多样化与环境变化无关。数据表明,在香草的​​可能起源中心,存在植物化学多态性,这间接暗示了遗传多态性的存在,这对于设计育种计划以优化香草的原始基因库多样性的利用和保存至关重要桔梗。

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