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Characterization of American hazelnut (Corylus americana) accessions and Corylus americana × Corylus avellana hybrids using microsatellite markers

机译:使用微卫星标记鉴定美国榛子(Corylus americana)品种和Corylus americana×Corylus avellana杂种

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The American hazelnut (Corylus americana Marshall) is native to the eastern United States and Southern Canada. In the early decades of the last century, breeding work by several individuals attempted to combine the cold hardiness and disease resistance of the American hazelnut with the larger nut size of the European hazelnut (C. avellana L.). Many hybrid selections grown today trace back to these early efforts. Over the past three decades, representatives of C. americana were collected and are preserved in Corvallis, Oregon. Seeds were collected along roadsides, in hedgerows, in woodland clearings, and in gardens. The seeds were germinated and the best of the resulting seedlings were preserved for later use. In this study, genetic diversity was studied in 87 American and 67 hybrid hazelnut selections using 21 microsatellite loci. The total number of alleles was 266 in the 162 accessions examined, of which 229 were present in C. americana accessions, but only 168 in the Arbor Day Farm hybrids, and 116 in the hybrids of C. americana ‘Rush’. In the American accessions, a high level of genetic diversity was observed (H e = 0.74, H o = 0.68). A genetic similarity matrix of the American and hybrid accessions, and a few European cultivars, was constructed and the resulting dendrogram revealed seven major groups: European cultivars and ‘Rush’ hybrids, two groups of Arbor Day Farm hybrids, three groups of C. americana accessions, and a mixed group of hybrid and American accessions. Analysis confirmed the reported parentage of 10 hybrids. The genetic diversity among the American hazelnut accessions, untapped to date, will be useful in breeding hybrids that will allow expansion of hazelnut production into eastern North America.
机译:美国榛子(Corylus americana Marshall)原产于美国东部和加拿大南部。在上个世纪初的几十年中,几个人的育种工作试图将美国榛子的抗寒性和抗病性与欧洲榛子的更大的坚果大小结合在一起。今天生长的许多杂种选择可以追溯到这些早期的努力。在过去的三十年中,美国梭状芽胞杆菌的代表被收集并保存在俄勒冈州的科瓦利斯。在路边,树篱,林间空地和花园中收集种子。种子发芽,并保留最好的幼苗,以备后用。在这项研究中,使用21个微卫星基因座在87个美国榛子和67个杂交榛子品种中研究了遗传多样性。在所检查的162个种质中,等位基因总数为266,其中在美国C.种中存在229个,但是在Arbor Day Farm杂种中只有168个,在美国C.Rush杂种中只有116个。在美洲种中,观察到高水平的遗传多样性(H e = 0.74,H o = 0.68)。构建了美洲和杂种的遗传相似性矩阵,以及一些欧洲品种,得到的树状图显示了七个主要类别:欧洲品种和“ Rush”杂种,两组Arbor Day Farm杂种,三组美洲种。加入,以及混合的杂种和美国的杂种。分析证实了10种杂种的亲缘关系。迄今尚未开发的美国榛子种之间的遗传多样性将有助于杂交种的培育,从而使榛子的生产扩展到北美东部。

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