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Genetic diversity of Spanish Cucurbita pepo landraces: an unexploited resource for summer squash breeding

机译:西班牙南瓜pepo地方品种的遗传多样性:夏南瓜育种未开发的资源

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摘要

Cucurbita pepo is a worldwide cultivated vegetable of American origin. Most of the widely grown commercial types are known as summer squashes and belong to the elongated forms of C. pepo ssp. pepo (Cocozelle, Vegetable marrow and Zucchini groups). These forms were developed in Europe after the arrival of the first American landraces through a process of selection and fixation that led to a loss of genetic diversity. Part of the genetic variability of the first American cultigens remains intact in diverse landraces that are still cultivated for self-consumption and sale in local markets. Using the first collection of genomic and EST-derived microsatellites that has just become available for the species, we compared the natural variation present in a collection of Spanish landraces with that of a set of commercial varieties and hybrids, representing current summer squash market offerings. A total of 194 alleles allowed us to distinguish all the genotypes, even those that were closely related. In general, Cocozelle and Vegetable marrow, groups with considerably long histories, were more variable than the Zucchini group, of more recent origin. We found significant genetic diversity among landraces. The variation present among landraces belonging to the Zucchini group was larger than that of the commercial cultivars. Cluster, principal coordinate and population structure results suggested that the variation of the Spanish landraces has not been extensively used in breeding. Commercial summer squashes can therefore benefit from this underexploited variability, especially from certain landraces that already display favourable commercial traits.
机译:南瓜属是一种世界范围内种植的美国起源的蔬菜。大多数广泛种植的商业类型被称为夏南瓜,属于拉美梭菌(C. pepo ssp)的细长形式。 pepo(可可,蔬菜骨髓和西葫芦组)。这些形式是在第一个美国地方物种到达欧洲后通过选择和固定过程导致遗传多样性丧失的欧洲发展起来的。首批美国品种的遗传变异的一部分在各种地方品种中仍然完好无损,这些地方品种仍在当地供人们自用和销售。使用刚刚可供该物种使用的第一组基因组和EST衍生微卫星,我们将西班牙地方品种集合中的自然变异与一组商业品种和杂种的自然变异进行了比较,代表了当前的西葫芦市场产品。总共194个等位基因使我们能够区分所有基因型,甚至那些密切相关的基因型。通常,历史悠久的可可松和蔬菜骨髓组比起最近的西葫芦组具有更大的可变性。我们发现地方种之间存在显着的遗传多样性。属于西葫芦组的地方品种之间存在的差异大于商业品种。聚类,主要坐标和种群结构结果表明,西班牙地方品种的变种尚未广泛用于育种。因此,商业夏季南瓜可以从这种未被充分利用的可变性中受益,特别是受益于某些已经显示出良好商业特性的地方品种。

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