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Inter-MITE polymorphisms of a newly identified MITE show relationships among sugarcane (Saccharum) species

机译:新鉴定的MITE的MITE间多态性显示甘蔗(Saccharum)物种之间的关系

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Sugarcane, one of the most important tropical crops, belongs the genus Saccharum. This genus consists of six species, four cultivated and two wild. The domestication histories of the four cultivated Saccharum species is an interesting and important topic of study. Previous studies have categorized the four cultivated species into two groups, one consisting only of S. edule and the other comprising S. officinarum, S. sinense and S. barberi. All four species have inherited the genomic DNA of S. robustum, one of two wild relative species. Saccharum species have large genomes with complex structures, as evidenced by chromosomes with a high degree of polyploidy, alloploidy and aneuploidy. Miniature inverted-repeat transposable elements (MITEs) are class II (DNA) transposons that disperse throughout the plant genome. In this study, a Tourist family MITE sequence with 18/19-bp terminal inverted repeats and a 2-bp target site duplication was newly identified from genomic DNA of S. robustum. The abundant accumulation of this MITE sequence in the sugarcane genome enabled the application of inter-MITE polymorphism (IMP) analysis to Saccharum. IMP analysis revealed the genetic relationships among all six Saccharum species and the domestication histories of the four cultivated species.
机译:甘蔗是最重要的热带作物之一,属于蔗糖属。该属包括六个物种,其中四个是栽培物种,两个是野生物种。四种栽培的蔗糖的驯化历史是一个有趣而重要的研究课题。先前的研究已将这四个栽培种分为两类,一组仅由小菜链球菌组成,另一组包括沙门氏菌,中华链球菌和巴氏链球菌。这四个物种都继承了鲁棒链霉菌的基因组DNA,这是两个野生近缘种之一。糖种具有庞大的基因组,具有复杂的结构,这由具有高度多倍性,同种异体性和非整倍性的染色体证明。微型反向重复转座因子(MITE)是II类(DNA)转座子,分散在整个植物基因组中。在这项研究中,从健壮链球菌的基因组DNA中新鉴定了具有18 / 19-bp末端反向重复和2-bp靶位点重复的Tourist家族MITE序列。该MITE序列在甘蔗基因组中的大量积累使MITE间多态性(IMP)分析可应用于蔗糖。 IMP分析揭示了所有六个糖种之间的遗传关系以及四个栽培种的驯化历史。

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