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首页> 外文期刊>Genetic Resources and Crop Evolution >Diversity of culturable bacteria and occurrence of phytopathogenic species in bean seeds (Phaseolus vulgaris L.) preserved in a germplasm bank
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Diversity of culturable bacteria and occurrence of phytopathogenic species in bean seeds (Phaseolus vulgaris L.) preserved in a germplasm bank

机译：种质库中保存的豆类种子（菜豆）中可培养细菌的多样性和致病物种的发生

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The preservation of plant genetic resources involves the conservation of the microbial biota associated with them. The presence of culturable bacteria in a series of 16 bean seed batches, corresponding to nine local bean varieties, stored in a germplasm bank was studied by amplifying and sequencing the 16S rDNA. Microorganisms identified in seed lots were classified into three groups: environmental biota (present in all samples), biota characteristic of humans and animals (present in 53 % of samples) and phytopathogenic biota (present in 19 % of samples). Genus diversity ranged between 0.6931 and 2.0942 according to the Shannon–Weaver Index (H’), the sample presenting the highest number of plant pathogenic bacteria being the most diverse. This result suggests that contrary to common practice in diagnostic laboratories, it is necessary to identify all culturable bacteria isolates from each sample. In addition, the fact that potentially phytopathogenic bacteria have been preserved in a genebank should emphasize the importance of rigorous sanitary controls for plant genetic resources.
机译：植物遗传资源的保存涉及与之相关的微生物群落的保存。通过对16S rDNA进行扩增和测序，研究了保存在种质库中的一系列16种豆种子批次（对应于9种本地豆品种）中可培养细菌的存在。在种子批次中鉴定出的微生物可分为三类：环境生物群（所有样品中都存在），人和动物的生物群特征（占53％样品中）和植物致病性生物群（占19％样品中）。根据香农-韦弗指数（H'），属多样性范围在0.6931至2.0942之间，样品中植物病原菌数量最多，种类最多。该结果表明，与诊断实验室的常规做法相反，有必要从每个样品中鉴定出所有可培养的细菌分离株。此外，潜在的致病细菌已被保存在种质库中这一事实应强调对植物遗传资源进行严格卫生控制的重要性。

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