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首页> 外文期刊>Genetic Resources and Crop Evolution >Phylogenetic characterisation of Onobrychis species with special focus on the forage crop Onobrychis viciifolia Scop.
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Phylogenetic characterisation of Onobrychis species with special focus on the forage crop Onobrychis viciifolia Scop.

机译：Onobrychis物种的系统发育特征，特别关注饲草作物Onobrychis viciifolia Scop。

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Sainfoin (Onobrychis viciifolia Scop.) is a perennial forage legume that possesses beneficial properties in the context of sustainable agriculture. In order to initiate a pre-breeding programme, we have assembled a germplasm collection of O. viciifolia from many geographic regions, as well as a collection of Onobrychis species that might be crossed with O. viciifolia to improve its biological and agronomic properties. The objective of this study was to obtain DNA sequences from a representative sample of these accessions in order to characterise their genetic diversity and to assess the robustness of the Onobrychis taxonomy. Because of potential problems with copurification of tannins and polyphenols from Onobrychis leaf tissue, three methods for DNA extraction were assessed and the most appropriate one identified. DNA sequences were obtained for the nuclear internal transcribed spacer region and the trnH-psbA and trnT-trnL intergenic spacers of the chloroplast genome. Neighbour joining trees were constructed and accessions were assigned to operational taxonomic units. The results indicated that there is substantial genetic diversity among Onobrychis species. Redundancies in species nomenclature were identified, as well as possible overlap between some of the sections into which the Onobrychis genus has been divided. A genetic distinction was apparent between O. viciifolia accessions from western Europe and those from eastern Europe and Asia, reflecting a similar division based on agronomic properties. Recognition of these different though related germplasms will be valuable in the design of breeding programmes for the rational improvement of sainfoin as a forage crop for sustainable agriculture.
机译：皂素（Onobrychis viciifolia Scop。）是多年生牧草，在可持续农业的背景下具有有益的特性。为了启动预育计划，我们收集了来自许多地理区域的薇薇叶草的种质资源，以及可能与薇薇叶草杂交的Onobrychis物种，以改善其生物学和农艺特性。这项研究的目的是从这些登录品的代表性样品中获得DNA序列，以表征其遗传多样性并评估Onobrychis分类学的稳健性。由于从Onobrychis叶片组织中单宁和多酚共纯化的潜在问题，评估了三种DNA提取方法，并确定了最合适的一种。获得了核内转录间隔区和叶绿体基因组的trnH-psbA和trnT-trnL基因间隔区的DNA序列。构造了邻居合并树，并将种质分配给了操作分类单位。结果表明，Onobrychis物种之间存在大量的遗传多样性。确定了物种命名的冗余，以及将Onobrychis属划分为的某些部分之间可能的重叠。来自西欧和来自东欧和亚洲的vic.folia叶植物种之间存在遗传差异，这反映了基于农艺特性的相似划分。认识到这些不同但相关的种质将在设计育种计划中合理使用sainfoin作为可持续农业的饲料作物方面具有重要价值。

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