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首页> 外文期刊>Genetic Resources and Crop Evolution >Genetic diversity in wild and cultivated black raspberry (Rubus occidentalis L.) evaluated by simple sequence repeat markers
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Genetic diversity in wild and cultivated black raspberry (Rubus occidentalis L.) evaluated by simple sequence repeat markers

机译：用简单的序列重复标记评估野生和栽培黑莓（Rubus occidentalis L.）的遗传多样性

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摘要

Breeding progress in black raspberry (Rubus occidentalis L.) has been limited by a lack of genetic diversity in elite germplasm. Black raspberry cultivars have been noted for showing very few phenotypic differences and seedlings from crosses between cultivars for a lack of segregation for important traits. Despite these challenges, little molecular work has been done to explore genetic diversity and relationships in wild and cultivated black raspberry germplasm. Microsatellite, or simple sequence repeat (SSR), markers are highly polymorphic codominant markers useful for studying genetic diversity, population genetics, genetic fingerprinting and other applications. We examined genetic diversity in 148 wild and cultivated black raspberry accessions using 21 polymorphic SSR markers. Black raspberry cultivars clustered tightly and showed higher than expected heterozygosity while that of wild accessions was low. Relationships between wild black raspberry accessions were poorly resolved and regional clusters were mostly absent from our analysis. Our results indicated that wild black raspberry germplasm is a relatively untapped resource available for future breeding.
机译：黑树莓（Rubus occidentalis L.）的育种进展受到精英种质缺乏遗传多样性的限制。黑树莓品种因缺乏重要特征的分离而显示出很少的表型差异和品种间杂交产生的幼苗。尽管存在这些挑战，但是在野生和栽培的黑树莓种质资源中探索遗传多样性和相互关系的分子研究很少。微卫星或简单序列重复（SSR）标记是高度多态性显性标记，可用于研究遗传多样性，种群遗传学，遗传指纹图谱和其他应用。我们使用21个多态性SSR标记检查了148个野生和栽培黑树莓种质的遗传多样性。黑树莓品种紧密聚集，表现出高于预期的杂合度，而野生种则较低。在我们的分析中，野生黑树莓种质之间的关系解析较差，并且大多没有区域簇。我们的结果表明，野生黑树莓种质资源是一个相对未开发的资源，可用于将来的育种。

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  • 来源
    《Genetic Resources and Crop Evolution》 |2012年第8期|p.1849-1865|共17页
  • 作者

    Michael Dossett; Nahla V. Bassil; Kim S. Lewers; Chad E. Finn;

  • 作者单位

    Corvallis, OR, 97331, USA;

    US Department of Agriculture, Agricultural Research Service, National Clonal Germplasm Repository, 33447 Peoria Road, Corvallis, OR, 97333, USA;

    US Department of Agriculture, Agricultural Research Service, Fruit Laboratory, 10300 Baltimore Avenue, BARC-West Building 010A, Beltsville, MD, 20705, USA;

    US Department of Agriculture, Agricultural Research Service, Horticultural Crops Research Unit, 3420 NW Orchard Avenue, Corvallis, OR, 97330, USA;

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  • 正文语种 eng
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  • 关键词

    Blackcap; Microsatellite; Molecular markers; Rubus occidentalis; SSR;

    机译：黑帽;微卫星;分子标记;西洋参;SSR;

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