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首页> 外文期刊>Genetic Resources and Crop Evolution >Set up of simple sequence repeat markers and first investigation of the genetic diversity of West-African watermelon (Citrullus lanatus ssp. vulgaris oleaginous type)
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Set up of simple sequence repeat markers and first investigation of the genetic diversity of West-African watermelon (Citrullus lanatus ssp. vulgaris oleaginous type)

机译：建立简单的序列重复标记并首次调查西非西瓜（Citrullus lanatus ssp。vulgaris oleaginous type）的遗传多样性

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摘要

Citrullus lanatus ssp. vulgaris oleaginous type (West-African watermelon) is a crop cultivated in sub-Saharan Africa for its dried seeds reported to be rich in nutrients. In previous studies, little polymorphism was found in watermelon—cultivated for its flesh with the use of microsatellite (SSR) markers. Such study has never been applied to the oleaginous type until now. The objectives of the present study were firstly to apply the SSR markers set up for watermelon to the West-African watermelon and secondly to study the genetic structure of this type in Ivory Coast. For the first objective, 37 markers were studied on eight plants pertaining to four accessions. For the second objective, the polymorphic markers were applied on three morphologically and geographically separated accessions with twenty plants per accession. Multiple correspondence analysis (MCA), unweighted pair-group method with arithmetic averaging (UPGMA), molecular analysis of variance (AMOVA) and assignments test structure were applied. The optimal annealing temperature varied from 49 to 59°C according to the markers. Thirty-two markers that proved to amplify their respective loci were selected, but only nine of them appeared to show polymorphism on the set of 8 plants studied. The application of these markers on the three accessions revealed several features. No stucturation into sub-populations was observed inside a given accession. The genetic variance proved to be substantially higher between the different accessions than inside a given accession. Moreover this analysis is a first hint that the morphology classification does not match the genetic structure of C. lanatus. The results of this work provide the first quantitative information regarding the genetic variability of Citrullus lanatus oleaginous type. In order to sharpen our understanding of the mechanisms responsible for the genetic variance inter/intra accessions, further studies based on a larger sample of plants and accessions are required.
机译：柑桔寻常的含油类型（西非西瓜）是撒哈拉以南非洲种植的一种农作物，据报道其干种子富含营养。在先前的研究中，在西瓜中几乎没有发现多态性-通过使用微卫星（SSR）标记将其以果肉栽培。迄今为止，这种研究从未应用于油性类型。本研究的目的是首先将为西瓜建立的SSR标记应用于西非西瓜，其次研究象牙海岸这种类型的遗传结构。为了第一个目标，研究了八种植物（共四个种质）的37个标记。为了第二个目的，将多态性标记物应用于在形态和地理上分离的三个种质，每个种质20个植物。应用多重对应分析（MCA），算术平均的非加权成对分组法（UPGMA），方差分子分析（AMOVA）和作业测试结构。最佳退火温度根据标记在49至59°C之间变化。选择了32个被证明可以扩增其各自基因座的标记，但只有9个在所研究的8株植物上表现出多态性。这些标记物在三个种上的应用揭示了几个特征。在给定的种质内未观察到亚群的结构。事实证明，不同种质之间的遗传变异比给定种质内部的显着更高。此外，该分析是形态分类与C. lanatus的遗传结构不匹配的第一个提示。这项工作的结果提供了有关油柑柑桔型遗传变异的第一个定量信息。为了加深我们对造成基因间/种内遗传变异的机制的理解，需要基于更大的植物和种质样本进行进一步的研究。

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