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首页> 外文期刊>Genetic Resources and Crop Evolution >Comparison of the efficiency of A–PAGE and SDS–PAGE, ISSRs and RAPDs in resolving genetic relationships among Triticum and Aegilops species
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Comparison of the efficiency of A–PAGE and SDS–PAGE, ISSRs and RAPDs in resolving genetic relationships among Triticum and Aegilops species

机译：比较A-PAGE和SDS-PAGE，ISSR和RAPD解决小麦与埃格种属之间的遗传关系的效率

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摘要

Species that belong to the genus Triticum L. and the genetically related Aegilops L. genus are important genetic and economic resources because they have an evolutionary relationship with the two main agricultural crops T. aestivum (bread wheat) and T. durum (durum wheat). Therefore, it is important to understand the genetic relationships among the cultivated wheat species and their wild relatives. The latter have a great role in the improvement of cultivated wheat. Molecular markers are the best choice and most reliable means to study these relationships accurately. In this study, we compared the efficiency of the biochemical methods A–PAGE and SDS–PAGE on seed storage proteins and the molecular methods RAPDs and ISSRs to explore the genetic relationships among seven species of Triticum and 20 Aegilops species. Three phylogenetic trees obtained in this study were compared with available classifications and phylogenetic trees constructed earlier for these species. It was noted that the tree based on ISSRs data was the most congruent with those classification and trees. This may be attributed to the fact that ISSRs is more specific, and therefore more reliable. This study is the first to study genetic relationships among all species studied here using biochemical and molecular techniques.
机译：属于小麦属和遗传相关的埃吉洛斯属的物种是重要的遗传和经济资源，因为它们与两种主要农作物T. aestivum（面包小麦）和T. durum（硬质小麦）具有进化关系。 。因此，重要的是了解栽培小麦品种及其野生近缘种之间的遗传关系。后者在改良栽培小麦方面具有重要作用。分子标记是准确研究这些关系的最佳选择和最可靠的方法。在这项研究中，我们比较了生化方法A–PAGE和SDS–PAGE对种子贮藏蛋白的效率，以及分子方法RAPD和ISSR的效率，以探索7种小麦和20种埃及盾草之间的遗传关系。将本研究中获得的三棵系统树与可用的分类进行比较，并为这些物种建立较早的系统树。需要指出的是，基于ISSRs数据的树与那些分类和树最一致。这可能归因于以下事实：ISSR更具体，因此更可靠。这项研究是首次使用生化和分子技术研究这里研究的所有物种之间的遗传关系。

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