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首页> 外文期刊>Genetic Resources and Crop Evolution >Analysis of Genetic Diversity in Colonial Bentgrass (Agrostis capillaris L.) using Randomly Amplified Polymorphic DNA (RAPD) Markers
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Analysis of Genetic Diversity in Colonial Bentgrass (Agrostis capillaris L.) using Randomly Amplified Polymorphic DNA (RAPD) Markers

机译：利用随机扩增多态性DNA（RAPD）标记分析殖民地Bentgrass（Agrostis capillaris L.）的遗传多样性

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摘要

Colonial bentgrass (Agrostis capillaris L.) is a cool-season grass, native to temperate Asia and Europe. It has good tolerance to low temperatures and partial shade and is well suited to golf course fairways and tees. Little information is available regarding levels and patterns of genetic variation among populations of colonial bentgrass, which would be useful for breeding programs. To study the genetic relationships among 27 colonial bentgrass accessions obtained from the US National Plant Germplasm System (NPGS), randomly amplified polymorphic DNA (RAPD) markers were scored and analyzed. Out of 80 primers screened, 16 were selected for further analysis, which yielded a total of 120 polymorphic bands used to differentiate the accessions. Dice's similarity coefficients for pair-wise comparisons ranged from 0.23 to 0.84 based on the RAPD data. Since there was no similarity coefficient value close to 1 between any two accessions, there was no apparent duplication among the sampled accessions. A dendrogram constructed on the basis of the Unweighted Pair Group Method with Arithmetic average (UPGMA) clustering algorithm clearly separated 26 of the accessions into three clusters with one accession distinct from the rest. The least similar pair of accessions was PI 204397 from Turkey and PI 628720 from Bulgaria, and the most similar pair was PI 509437 from Romania and PI 491264 from Finland. Clustering patterns based on principal components analysis (PCA) corresponded well with the dendrogram. A high cophenetic correlation (r = 0.82) was found between the RAPD data matrix and cophenetic matrix. The accession PI 628720, from Bulgaria, did not cluster with any other accessions.
机译：殖民地草（Agrostis capillaris L.）是一种凉爽的草，原产于亚洲和欧洲的温带。它对低温和部分阴影具有良好的耐受性，非常适合高尔夫球场球道和T恤。关于殖民地原生草种群间遗传变异的水平和模式的信息很少，这对育种计划很有用。为了研究从美国国家植物种质系统（NPGS）获得的27种殖民地弯曲草种质之间的遗传关系，对随机扩增的多态性DNA（RAPD）标记进行了评分和分析。在筛选出的80个引物中，选择了16个用于进一步分析，从而产生了总共120个多态性条带，用于区分这些种质。根据RAPD数据，用于成对比较的骰子相似系数在0.23至0.84之间。由于在任何两个登录号之间没有相似系数值接近1，因此在采样的登录号之间没有明显的重复。根据具有算术平均的非加权对群方法（UPGMA）聚类算法构建的树状图将26个种质清楚地分为三个簇，其中一个种质与其余种质不同。相似度最低的一对是土耳其的PI 204397和保加利亚的PI 628720，最相似的是罗马尼亚的PI 509437和芬兰的491264。基于主成分分析（PCA）的聚类模式与树状图非常吻合。在RAPD数据矩阵和同情矩阵之间发现高度同义相关（r = 0.82）。来自保加利亚的保藏号PI 628720与任何其他保藏号均未聚在一起。

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