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首页> 外文期刊>Genetic Resources and Crop Evolution >Identification of duplicates and fingerprinting of primary and secondary wild annual Cicer gene pools using AFLP markers
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Identification of duplicates and fingerprinting of primary and secondary wild annual Cicer gene pools using AFLP markers

机译：使用AFLP标记鉴定一级和二级野生年度Cicer基因库的重复项和指纹图谱

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摘要

Wild annual Cicer gene pools contain valuable germplasm for chickpea improvement programs. Previous research showed that duplication might exist in accessions collected from these gene pools, which would hinder chickpea breeding and related research. AFLP (amplified fragment length polymorphism) markers were used to fingerprint the world collections of the primary and secondary gene pools including C. reticulatum Lad., C. bijugum K.H. Rech., C. judaicum Boiss. and C. pinnatifidum Jaub. et Sp. Duplicates were detected in a total of 24 accessions in both the gene pools, highlighting the necessity to fingerprint the germplasm. Genotypic difference was detected as gene pool specific, species specific and accession specific AFLP markers. These were developed into fingerprinting keys for accession identification between and within species and gene pools. Use of AFLP markers to detect duplicates and to identify accessions is a reliable method which will assist in the characterisation and use of wild annual Cicer germplasm in chickpea improvement programs. We recommend the procedure presented in this paper as a standard approach for the precise genetic identification and characterisation of future world collections of wild Cicer, to keep germplasm integrity and to benefit chickpea breeding and related research programs.
机译：年度野生Cicer基因库包含用于鹰嘴豆改良计划的宝贵种质。先前的研究表明，从这些基因库收集的材料中可能存在重复，这会阻碍鹰嘴豆的繁殖和相关研究。 AFLP（扩增的片段长度多态性）标记被用于指纹识别主要和次要基因库的世界集合，包括网纹衣藻，比氏假丝酵母。 Rech。，C. judaicum Boiss。和C. pinnatifidum Jaub。等在两个基因库中总共检测到24个重复项，这突出显示了对种质进行指纹识别的必要性。基因型差异被检测为基因库特异性，物种特异性和种质特异性AFLP标记。这些被开发为指纹识别密钥，用于物种和基因库之间和内部的登录鉴定。使用AFLP标记来检测重复项并鉴定种质是一种可靠的方法，将有助于在鹰嘴豆改良计划中表征和使用野生年度Cicer种质。我们建议将本文介绍的程序作为标准方法，用于准确的遗传鉴定和表征未来野生野生世界的集合，以保持种质完整性并有利于鹰嘴豆育种和相关研究计划。

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  • 来源
    《Genetic Resources and Crop Evolution》 |2007年第3期|519-527|共9页
  • 作者

    F. Shan; H. J. Clarke; G. Yan; J. A. Plummer; K. H. M. Siddique;

  • 作者单位

    Centre for Legumes in Mediterranean Agriculture Faculty of Natural and Agricultural Sciences The University of Western Australia 35 Stirling Hwy 6009 Crawley WA Australia;

    Centre for Legumes in Mediterranean Agriculture Faculty of Natural and Agricultural Sciences The University of Western Australia 35 Stirling Hwy 6009 Crawley WA Australia;

    Centre for Legumes in Mediterranean Agriculture Faculty of Natural and Agricultural Sciences The University of Western Australia 35 Stirling Hwy 6009 Crawley WA Australia;

    Centre for Legumes in Mediterranean Agriculture Faculty of Natural and Agricultural Sciences The University of Western Australia 35 Stirling Hwy 6009 Crawley WA Australia;

    Centre for Legumes in Mediterranean Agriculture Faculty of Natural and Agricultural Sciences The University of Western Australia 35 Stirling Hwy 6009 Crawley WA Australia;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    AFLP; Chickpea; Cicer bijugum; Cicer judaicum; Cicer pinnatifidum; Cicer reticulatum; Duplicate detection; Germplasm; Legume;

    机译：AFLP;鹰嘴豆;Bicer bijugum;鹰嘴豆judaicum;鹰嘴豆pinnatifldum;网状Cicer;双倍检测;种质;豆类;

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